高效毛细管电泳场放大富集法测定中成药中的士的宁  

Determination of strychnine in Chinese patent medicine by field-amplified capillary electrophoresis

作　　者：周晓琴[1] 吴敏[1] 王竞[1] 孙成均[2] 钱广生[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川大学华西公共卫生学院,四川成都610041

出　　处：《华西药学杂志》2013年第5期509-511,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家"十一五"科技支撑计划课题资助(课题号:2006BAD27B02)

摘　　要：目的 提高毛细管电泳法的灵敏度,建立测定中成药中微量士的宁的高效毛细管电泳法.方法 采用高效毛细管电泳场放大富集法,运行缓冲液为70 mmol· mL-硼砂-乙腈（83：17,pH5.5）,10 kV电动进样,进样时间16 s,分离电压25 kV,分离温度25 c℃,检测波长205 nm.结果 士的宁0.2～16.0 μg·mL-1与峰面积的线性关系良好（r=0.9987）,检测限为0.022μg·mL-1.结论 所用方法准确、简便,有效提高了毛细管电泳法的灵敏度.OBJECTIVE To improve the sensitivity of capillary electrophoresis and establish an HPCE method for determination of the trace strychnine in Chinese patent medicine. METHODS Field - amplified capillary electrophoresis was used with the running buffer of 70 mmol·mL-1 boric acid- acetonitrile（83 ： 17）（pHS. 5） and an applied voltage of 25 kV at 25℃. The detection was performed with diode array detection at 205 nm. The sample was eventually diluted by ultra - pure water and injected for 16 s in electrokinetic type. RESULTS The linearity of strychnine was obtained over the rang of 0.2～16.0 μg·mL-1 （ r = 0. 9987 ） and the detection limit was 0.022μg·mL-1. CONCLUSION The method is simple,sensitive and effectively improved the sensitivity of capillary electroDhoresis.

关 键 词：高效毛细管电泳场放大 中成药 士的宁 

分 类 号：R917[医药卫生—药物分析学]