温阳健脾法治疗大鼠泻药性便秘的实验研究  

Therapy of warming yang and invigorating spleen for treating laxatives constipation in rats

作　　者：刘仍海[1] 刘薇[1] 

机构地区：[1]北京中医药大学东方医院肛肠科,北京100078

出　　处：《北京中医药大学学报》2013年第9期599-602,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30973755)

摘　　要：目的观察中医温阳健脾法对便秘的治疗作用及机理,以及对实验动物结肠c-kit、SCF蛋白表达的影响。方法运用泻药法复制便秘的大鼠动物模型,采用温阳健脾的中药配方颗粒(生白术/肉苁蓉2∶1)治疗,分成大剂量(9 g/kg)、中剂量(4.5 g/kg)、小剂量(2.25 g/kg),疗程均为4周,并与西药莫沙比利对照。观察大鼠的粪便粒数、重量和形态,采用活性炭悬液推进法,进行肠道运输功能检查,Western-blot方法检测实验大鼠结肠c-kit蛋白、SCF蛋白表达。结果温阳健脾法治疗便秘大鼠有明显疗效,对粪便粒数、重量和形态及肠道运输功能均有改善,与模型组对比有显著差异。温阳健脾法对便秘大鼠c-kit蛋白、SCF蛋白表达的实验结果表明,模型组表达明显低于正常组,差异有统计学意义,治疗后各组c-kit蛋白、SCF蛋白表达有所上升,c-kit蛋白表达治疗组与模型组比,有统计学意义,SCF蛋白表达各治疗组均有所上升,均高于模型组,但无明显统计学意义,可能与样本较少有关。结论温阳健脾法治疗泻药性便秘有明显疗效,泻药性便秘大鼠发生的机理可能与c-kit、SCF蛋白表达下降有关;温阳健脾法可以提高c-kit、SCF蛋白表达。Objective To observe the curative effect and mechanism of TCM therapy of warming yang and invigorating spleen on constipation, and its influences on the expressions of colon c-kit protein and SCF protein in rats. Methods The rat model of constipation was established by using laxatives, and treated with the formula granules of Chinese medicinals with actions of warming yang and invigorating spleen for 4 weeks, in which the proportion of Baizhu （Rhizoma Atractylodis Macrocephalae ） to Roucongrong （Herba Cistanches） was 2 to 1. The rats were divided into high-dose group （9 g/kg）, mid-dose group （4.5 g/kg） and low-dose group （2.25 g/kg）, and compared with mosapride group. The quantity, weight and form of feces of rats in all groups were observed, the intestinal transport function was detected by using activated carbon suspension propulsion method, and the expressions of colon c-kit protein and SCF protein in rats were detected by using Western blot test. Results The therapy of warming yang and invigorating spleen had an obvious curative effect on rats with constipation （quantity, weight and form of feces and intestinal transport function）, and showed significant differences when compared with model group. The experimental results indicated that the expressions of c-kit protein and SCF protein were significantly lower in model group than those in normal group with statistical significance, and increased in all treatment groups after treatment. The difference in the expression of c-kit protein was statistically significant between treatment groups and model group. The expressions of SCF protein increased in all treatment groups and were higher than that in model group, but the difference was not statistically significant, which might be related to the small sample size of the study. Conclusion The mechanism of laxative constipation in rats may be related to the decreased expressions of c-kit protein and SCF protein. The therapy of warming yang and invigorating spleen has an obvious curative effe

关 键 词：泻药性便秘 温阳健脾法 c—kit蛋白 SCF蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]