姜黄素对9月龄AD双转基因小鼠海马超微结构的影响  

Influence of curcumin on hippocampus ultrastructure in APPswe/PS1dE9 double transgenic mice (9 months old)

作　　者：樊惠[1] 魏鹏[1] 陈晓培[1] 冯慧利[1] 任映[1] 孙海芸[1] 杨金铎[1] 王蓬文[1] 

机构地区：[1]北京中医药大学东直门医院中药药理学实验室国家中医药管理局三级实验室中医内科学教育部重点实验室和北京市重点实验室北京100700

出　　处：《北京中医药大学学报》2013年第9期608-611,I0003,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81073076);教育部高等学校创新团队项目(No.IRT0810)

摘　　要：目的观察阿尔茨海默病(AD)模型APPswe/PS1dE9双转基因小鼠海马CA1区神经元以及突触的变化,评价姜黄素对APPswe/PS1dE9双转基因小鼠认知功能的影响。方法将9月龄的APPswe/PS1dE9双转基因小鼠随机分为模型组,罗格列酮组(10 mg/kg),姜黄素大剂量(400 mg/kg)、中剂量(200 mg/kg)、小剂量(100 mg/kg)组,每组6只;并以同月龄遗传背景相同的C57/BL6J小鼠作为对照组。每天灌胃给药1次,连续灌胃6个月。应用透射电子显微镜进行超微结构检测。结果电镜检测,对照组海马神经元和神经毡内结构均基本正常。模型组小鼠海马CA1区神经元固缩、电子密度升高,胞浆内脂褐素、脂滴明显增多,海马CA1区神经元出现程度不等的退行性变;神经毡内可见髓鞘变性,突触数量减少、结构异常,突触小泡聚集增多。姜黄素治疗组与模型组相比,突触增加,并使上述变化有不同程度的改善。结论姜黄素能通过增加突触、改变APPswe/PS1dE9双转基因小鼠突触等超微结构的变化,有可能改善APPswe/PS1dE9双转基因小鼠的学习记忆能力。Objective To observe the changes of neurons and synapses in hippocampus CAI zone in amyloid protein precursor （APPswe）/presenilin （PSldE9） double transgenic mice （AD model）, and to review the influence of curcumin on the cognitive function in APPswe/PS1 dE9 double transgenic mice. Methods All APPswe/PSldE9 double transgenic mice （9 months old） were randomly divided into model group, rosiglitazone （10 mg/kg/d） group, and high-dose （400 mg/kg/d）, mid-dose （200 mg/ kg/d） and low-dose （100 mg./kg/d） curcumin groups （high-dose group, mid-dose group and low-dose group, each n = 6）, and C57/BL6J mice with the same age and genetic background were assigned into the normal control group. All groups, except normal group, were intragastrically given relevant medicinal respectively once a day for 6 months. The changes of hippocampus ultrastructure were observed with transmission electron microscope（TEM）. Results The structure of hippocampus neutrons and neuropils were normal in normal group. In model group, neuron pyknosis, higher electron density, increase of lipofuscin （LPF） and lipid droplet in cytoplasm, and degeneration of neutrons in varying degrees were observed in the hippocampus CA1 zone, and myelinic degeneration, decrease and structural abnormality of synapses, and increased aggregation of synaptic vesicles were observed in neuropils. In curcumin groups the number of synapses increased and the above mentioned changes were ameliorated compared with model group. Conclusion Curcumin may improve the learning and memory abilities in APPswe/ PSldE9 double transgenic mice through increasing the number of synapses and changing hippocampus ultrastructure.

关 键 词：姜黄素 双转基因 电镜 突触 小鼠 

分 类 号：R285.5[医药卫生—中药学]