MicroRNAs在急性心肌梗死早期诊断中的评价  

Evaluation of circulating MicroRNAs in early diagnosis of acute myocardial infarction

作　　者：万晓群[1] 李卫华[1] 谢强[1] 郑武扬[1] 张黎静[1] 周洁琼[1] 黄峥嵘[1] 

机构地区：[1]厦门大学附属第一医院心内科,361003

出　　处：《中华老年医学杂志》2013年第10期1073-1075,共3页Chinese Journal of Geriatrics

摘　　要：目的用荧光定量聚合酶链式反应（qRT—PCR）方法检测急性心肌梗死（AMI）患者血浆中的MicroRNAs水平，评价其在AMI早期诊断中的价值。方法选取AMI患者24例，对照组20例为研究对象，分别在症状发作后3h、6h、12h、24h、48h、72h检测血浆中microRNA1、microRNA-133a、microRNA-208a、microRNA-499的水平。结果miRNA-1在缺血性胸痛发作后3h血浆水平明显升高达到高峰，72h基本降至正常；miRNA-133a在6h后血浆水平明显升高，12h达到高峰，48h降至正常；miRNA-1和miRNA-133a表达水平与心肌肌钙蛋白I（cTnI）相关，miRNA-1的峰值出现在cTnI之前，miRNA-133a峰值与cTnI发生在同一时间。结论循环中升高的miRNA1、miRNA-133a可能是诊断早期AMI的一种有潜力的、新型的生物标志物。Objective To evaluate the importance of plasma MicroRNAs in early diagnosis of acute myocardial infarction （AMI）. Methods 24 patients with AMI as the test group and 20 healthy volunteers as the control group were enrolled in this study. Plasma levels of microRNA-1, microRNA-133a, microRNA-208a and microRNA-499 were detected by quantitative real time polymerase chain reaction （qRT-PCR） at 3 h,6 h,12 h,24 h,48 h,72 h after the onset of AMI. Results Plasma microRNA-1 level was greatly increased and reached the peak at 3 h after AMI, then was decreased gradually to normal level at 72 h after AMI. Plasma microRNA-133a level was significantly elevated at 6 h after AMI, reached peak at 12 h after AMI, then was decreased to normal level at 48 h after AMI. Plasma microRNA-1 and microRNA-133a levels were correlated with cTnI expression. The peak time of microRNA-1 was earlier than that of cTnI, while the peak time of microRNA-133a was the same as that of cTnI. Conclusions Increased circulating microRNA-1 and microRNA-133a may serve as potential and novel biomarkers for early diagnosis of AMI.

关 键 词：心肌梗死 微RNAS 

分 类 号：R542.22[医药卫生—心血管疾病]