孕期酒精暴露对视网膜发育及细胞分化的影响  

Influence of prenatal alcohol exposure on retinal development and cell differentiation

作　　者：席艳[1] 周洁[1] 孔维芳[1] 王强[1] 刘彬[1] 郑红[1] 邓锦波[1] 

机构地区：[1]河南大学神经生物研究所,开封475004

出　　处：《生理学报》2013年第5期505-512,共8页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China(No.30771140;31070952;U1204311)

摘　　要：本文旨在研究酒精暴露对视网膜的发育和细胞分化过程所造成的影响。建立C57BL/6J小鼠孕期酒精暴露模型,用HE染色和免疫荧光技术,选取P0到P30仔鼠观察视网膜的正常发育和细胞分化过程,及酒精对其造成的影响。结果显示,孕期酒精暴露可以诱导仔鼠视网膜片层化形成延迟、水平细胞和双极细胞的数量减少(P<0.05)和极性紊乱、视网膜增厚(P<0.01),具有酒精剂量依赖性(P<0.05)和酒精暴露停止后的长时程效应。以上结果提示,孕期酒精暴露可引起仔鼠视觉相关神经元的数量减少及形态异常,导致视觉系统的发育障碍。The aim of the present study was to investigate the effects of prenatal alcohol exposure （PAE） on the development and cell differentiation of retina in offspring. The mouse model of PAE was made. HE staining and immunofluorescent labeling were carried out to visualize the structure, development and cell differentiation of the retina from postnatal day 0 （P0）-P30 offspring. The results showed that PAE can lead to the retardation of retinal development, the reduction of number of bipolar cells and horizontal cells, the disorder of horizontal cells＇ polarity, as well as the retinal thickening in a dose-dependent manner. The data suggest that alcohol expo- sure during pregnancy can lead to the developmental retardation of retina and decreased number of bipolar cells and horizontal cells in the retina of offspring.

关 键 词：片层化 双极细胞 视网膜发育 水平细胞 胎儿酒精综合症 

分 类 号：R714.5[医药卫生—妇产科学]