带血管蒂膈肌瓣重建食管动物模型的制作  

Experimental animal model of pedicled diaphragmatic muscle flap for reconstruction of defected esophagus on pigs

作　　者：范义婷[1] 王武军[1] 王雪莲[2] 刘太省[1] 王昊飞[1] 

机构地区：[1]南方医科大学南方医院胸心血管外科,广州510515 [2]南方医科大学第三附属医院麻醉科,广州510630

出　　处：《中国临床解剖学杂志》2013年第5期568-571,共4页Chinese Journal of Clinical Anatomy

基　　金：南方医院院长基金(2009C003)

摘　　要：目的探索猪带蒂膈肌瓣重建食管动物模型的制作方法,对比以不同血管蒂的膈肌瓣重建食管的可行性及优劣,为进一步研究打下实验基础。方法健康长白猪10头,体质量24~25 kg;根据血管蒂的不同随机分2组,每组5例。A组,以左膈下动脉为蒂;B组,以左心包膈动脉为蒂,左侧膈神经同蒂血管一同游离。全麻下切除部分下段食管(3~5 cm),取带血管蒂膈肌瓣管状成形并重建缺损食管,缺损膈肌涤纶补片修补。结果 B组的生存时间及长期存活动物例数均大于A组(P<0.05);B组蒂血管心包膈动脉的游离长度显著大于A组膈下动脉的游离长度(P=0.004);B组膈肌瓣长度及宽度均大于A组可切取的长度(P=0.001)及宽度(P<0.001)。结论成功建立猪膈肌重建食管的动物模型,以心包膈动脉和以膈下动脉为蒂的膈肌瓣重建食管下段均是可行的;B组模型较A组模型更具优势。Objectivo This study aims to establish an animal model of diaphragmatic muscle flap with vascular pedicle for reconstruction of the esophagi in pigs, and compare the feasibilities, advantages and disadvantageso of using diaphragmatic muscle flaps with different vascular pedicles for reconstruction of the esophagusso as to lay an experimental foundation for further study. Molhod 10 healthy Landrace, weighing between 24 and 25 kg, were divided randomly into two groups （A and B） according to different vascular pedicles. Group A （n = 5）, left phrenic artery was used as the vascular pedicle. Group B（n = 5）, left pericardium phrenic artery with left phrenic nerve was used as thevascular pedicle. The lower 3-5 cm segment of the esophagi of the pigs in the two groups were cut off under general anesthesia, and diaphragmatic muscle flaps with vascular pedicles were harvested and rendered into tubes to reconstruct the defected esophagi. The then defected diaphragmatic muscles were repaired by dacron patch. Ro^mlt The survival time and the cases of long-term survival animals in group B were greater than group A （P 〈 0.05）; the isolated length of pericardium diaphragmatic arteries in group B was significantly greater than the isolated length of phrenic arteries in group A （P = 0.004）; the length and width of diaphragrnatic valves in Group B were greater than the feasible length （P =0.001） and width （P〈0.001） in group A. Conchmion The experimental animal model of pedicled diaphragmatic muscle flaps reconstructing defected esophagi on pigs has been successfully established. It is feasible for diaphragmatic muscle flaps with pericardium phrenic artery or phrenic artery to reconstruct esophagi; the model of group A has more advantages than group B.

关 键 词：猪 食管重建 带蒂膈肌瓣 心包膈动脉 膈下动脉 

分 类 号：R655.6[医药卫生—外科学]