人工关节磨损钛颗粒干扰rBMSCs的成脂分化能力  

Investigation on Disturbing of Adipocytic Differentiation of rBMSCs by Loaded Titanium Particles

作　　者：吴江[1,2] 赵志伟[1] 王艳霞[3] 陈槐卿[1] 

机构地区：[1]四川大学华西基础医学与法医学院,成都610041 [2]新疆医科大学基础医学院,乌鲁木齐830011 [3]四川大学图书馆,成都610041

出　　处：《生物医学工程学杂志》2013年第5期1010-1014,1021,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10802054;11262020);高等学校博士点基金资助项目(20020610049)

摘　　要：人工关节磨损钛颗粒可抑制大鼠骨髓间充质干细胞(rBMSCs)增殖、黏附以及成骨分化能力。为进一步探讨钛颗粒对rBMSCs成脂分化能力的影响,本实验采用组织细胞化学法、RT-PCR法观察3种直径(6.9、2.7和0.9μm)钛颗粒对rBMSCs成脂分化过程中脂肪滴形成、脂蛋白脂肪酶(LPL)mRNA表达和甘油三酯(TG)分泌的情况,比较不同直径钛颗粒对rBMSCs成脂分化能力的影响。结果发现3种钛颗粒对rBMSCs成脂分化能力的影响趋势并不相同:0.9μm和2.7μm,尤其是0.9μm钛颗粒可明显促进rBMSCs脂肪滴形成、LPL mRNA表达和TG分泌;而随着颗粒作用时间延长和浓度增加,6.9μm组细胞成脂分化能力受到抑制。结果表明,不同直径人工关节磨损钛颗粒可明显干扰rBMSCs的成脂分化能力,小颗粒促进rBMSCs的成脂分化,而大颗粒抑制rBMSCs的成脂分化。Our previous investigations demonstrated that varying sizes of loaded titanium particles could inhibit prolif- eraion, adhension and osteoblastic differentiation of rat bone marrow mesenchymal stem cells （rBMSCs）. The pres- ent study aims to validate the hypothesis that particled-shaped wear debris from prosthetic implants influence the adi- pocytic differentiation of rBMSCs. The effects of different sizes of loaded titanium particles （6.9ptm, 2.7μm and 0.9μm） on the adipocytic differentiation of rBMSCs were studied by observing lipid droplet formation, assaying the expression of lipoprotein lipase （LPL） mRNA by RT-PCR and Triglycerides （TG） secretion. The loaded titanium particles were found to influence adipogenesis of rBMSCs, but had different effects, depending upon particle size, concentration and loading time duration. 2.7 μm and 0.9 μm titanium particles promoted lipid droplet formation, LPL mRNA expression and TG secretion, while at a higher concentration of titanium particles and a longer loading duration, 6.9 μm titanium particles gradually inhibited adipogenic differentiation of rBMSCs. Three sizes of loading titanium particles obviously disturbed the adipocytic differentiation capability of rBMSCs： the smaller particles pro rooted but the larger inhibited the adipogenic differentiation of rBMSCs.

关 键 词：人工关节 无菌性松动 钛颗粒 骨髓间充质干细胞 

分 类 号：R318.08[医药卫生—生物医学工程]