裸鼹鼠肺脏显微结构与超微结构观察  

Observation on Micro-and Ultra-structures of the Lung in Naked Mole Rar （Heterocephalus glaber）

作　　者：袁子彦[1] 赵懿宁[2] 张璐[1] 赵善民[1] 林丽芳[1] 汤球[1] 孙伟[1] 余琛琳[1] 蔡丽萍[1] 徐晨[1] 崔淑芳[1] 

机构地区：[1]第二军医大学实验动物中心,上海200433 [2]上海市杨浦区精神卫生中心,上海200090

出　　处：《实验动物与比较医学》2013年第5期378-382,共5页Laboratory Animal and Comparative Medicine

基　　金：上海市科技发展基金项目（No.11140901700）与（No.12140900200）联合资助

摘　　要：目的 观察和分析裸鼹鼠肺脏的形态结构与组成.方法 采集裸鼹鼠肺脏经石蜡切片苏木精-伊红染色,然后光学显微镜下观察裸鼹鼠肺脏的显微结构,以及经冰冻超薄切片和染色后,通过透射电镜观察和分析裸鼹鼠心脏的超微结构.结果 光学显微镜观察发现裸鼹鼠肺脏有类似支气管扩张和肺不张等病理变化,而且肺血管分布情况和支气管上皮细胞的形态和分布与小鼠有很大不同;透射电镜观察裸鼹鼠肺脏并没有支扩和肺不张的病理变化,肺泡上皮细胞有微绒毛,裸鼹鼠肺脏气血屏障有增厚现象.结论 裸鼹鼠肺脏呈幼态性发育,而且肺泡可根据机体代谢率和需氧量的不同而进行开启和关闭.Objective To observe and analyze the the morphological structure and composition of lung in the naked mole rat （Heterocephalus glaber）. Method The lungs were isolated from anaesthetic animals and then made to paraffin sections, which were stained by hematoxytin-eosin （HE） dye. The microstructure of lung was observed under optical microscope. Meanwhile, the fresh lungs were pro- cessed to ultrathin sections and stained. At last, the ultramicroscopic structure of lungs was observed by transmission electron microscope. Result The lungs of NMR looks like bronchiectasis and pulmonary atelectasis observation through optical microscope. Besides, the distribution of vasculars and bronchial epithelial cells are quite different from mice. And, the surface of alveolar epithelial cells in NMR are rich in microvilli. Moreover, the blood-gas barrier of NMR is thiker then mice＇s. Conclusion The lungs of NMR show infantile development. More importantly, NMR can open or close alveolar according to the body＇s metabolic rate and oxygen demand.

关 键 词：裸鼹鼠 肺脏 显微结构 超微结构 

分 类 号：Q95-33[生物学—动物学]