滇西亚种树鼩心肌酶、部分肾功能指标的测定及其初步分析  

Measurement and Analysis of Myocardial Enzymes and Some Renal Function Indice in Tupaia Belangeri Chinensis

作　　者：郑永仁[1] 王礡 郑红 角建林[2] 刘梦思[4] 李进涛[2,3] 

机构地区：[1]云南中医学院实验中心,云南昆明650500 [2]昆明医科大学实验动物学部,云南昆明650500 [3]扬州大学兽医学院,江苏扬州225009 [4]昆明医科大学,临床医学专业云南昆明650031

出　　处：《昆明医科大学学报》2013年第10期5-9,共5页Journal of Kunming Medical University

基　　金：国家科技支撑计划项目(2009BAI83B02);云南省科技厅应用基础研究计划面上项目(2012FB155);云南省科技厅应用基础研究联合专项项目(2012FB022)

摘　　要：目的：为建立和评价人类疾病树鼩模型奠定生理生化基础．方法滇西亚种树鼩92只，雌雄各半．禁食12 h后，非麻醉状态下心脏采血0.8～1.0 mL装入灭菌离心管用于分离制备血清并用Olympus AU5400全自动生化分析仪进行生化指标测定．每只树鼩人工挤尿1.5～2.0 mL存于灭菌离心管用Combi-scan500尿液分析仪进行尿液分析测定．用SPSS统计软件对测定的数据统计分析并与人的参考值进行了比较．结果滇西亚种树鼩雌雄间心肌酶与部分肾功能指标测定值中乳酸脱氢酶、α-羟丁酸脱氢酶、肌酐、尿酸、尿比重、酸碱度比较差异有统计学意义（＜0.05），其余的指标差异无统计学意义（＞0.05）．滇西亚种树鼩雌雄合并、雌性和雄性心肌酶与部分肾功能指标测定值与人的参考值比较得出：尿素、尿比重、尿糖、尿胆原、尿胆红素、尿亚硝酸盐均在人的参考值范围内，乳酸脱氢酶、肌酸激酶、α-羟丁酸脱氢酶、肌酸激酶同工酶CKMB均高于人的参考值，肌酐、尿酸和尿酸碱度稍低于人的参考值．白细胞、尿蛋白、酮体、隐血多数与人的参考值一致为阴性，但偶有阳性的，阳性率分别为3.95%、46.1%、39.5%、2.63%．维生素C与人的参考值相反为阳性，但也偶有阴性的，阴性率为6.6%．结论尿素、尿比重、尿糖、尿胆原、尿胆红素、尿亚硝酸盐可直接作为人类疾病树鼩模型的评价指标，其它指标参数可作为判断树鼩正常生理生化的依据．Objective To lay the physiological and biochemical basis for establishing and evaluating tree shrews model of human disease. Methods There were 92 Tupaia belangeri chinensis, in which half of them were male,they were allowed to eat nothing for 12 hours, then we sampled heart blood 0.8~1.0 mL without anesthesia and put blood samples into sterilized centrifuge tube for separation and preparation of serum. Olympus AU5400 automatic biochemistry analyzer was used to measure biochemical indexes. Then 1.5~2.0 mL of urine of each tree shrew was collected and put into sterilized centrifuge tube for measuring renal function by using Combi-scan500 urine analyzer. Fianally SPSS statistics software was used to analyse the measured values, and comapared the measured values with the human reference values. Results There were significant differences in myocardial enzymes and some renal function indexes such as lactic dehydrogenase,α- hydroxybutyric, acid dehydrogenase, creatinine, uric acid, urine specific gravity and pH value between male and female Tupaia belangeri chinensis （ 〈0.05）, and there was no significant difference in the rest of the measured values （ 〉0.05） . Then determination value of Tupaia belangeri chinensis, male’s and female’s, myocardial enzyme and part of the renal function indexes were compared with the human reference values. Some indexes including urea, urine specific gravity, urine, urobilinogen were in the range of human reference value, while the values of urine bilirubin, urine nitrite. lactate dehydrogenase, creatine kinase, α-hydroxybutyrate dehydrogenase, creatine kinase isoenzyme CKMB were higher than the human reference value. White blood cell, urine protein, ketone, occult blood most of them were negative as the same to human reference value, but sometimes were positive, and the positive rates respectively were 3.95%and 46.1%,39.5%,2.63%. The measured value of Vitamin C was positive that is completely opposite to human reference value, but sometimes is negative, the negative ra

关 键 词：滇西亚种树鼩 心肌酶 肾功能 测定 分析 

分 类 号：R446.1[医药卫生—诊断学]