魔芋低聚甘露糖对SD大鼠的致畸试验  

Teratogenic toxicity of konjac mannan oligosaccharide on pregnant SD rats

作　　者：蒋敏[1] 罗春勤 史劲松[1] 

机构地区：[1]江南大学药学院制药工程实验室,江苏无锡214122 [2]成都永安缘和生物科技有限公司,四川成都611630

出　　处：《癌变．畸变．突变》2013年第5期377-379,383,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金："十二五"国家科技支撑计划项目(2012BAD33B06)

摘　　要：目的：探讨经甘露聚糖酶降解所得的魔芋低聚甘露糖对sD大鼠致畸敏感期的毒性。方法：将妊娠期SD大鼠，随机分成4组，每组12只，分别设魔芋低聚甘露糖3个剂量组（625、1250、2500mg／kg）和阴性对照组（蒸馏水）。妊娠敏感期（第7～16天）连续灌胃给药，第20天处死孕鼠，取出胎鼠，记录总着床数、活胎数、死胎数、吸收胎数等，称子宫连胎总质量、活胎质量，并量胎鼠身长，同时检查胎鼠外观、内脏和骨骼有无畸形。结果：与阴性对照组比较，魔芋低聚甘露糖各剂量组孕鼠体质量、活胎数、死胎数、吸收胎数、胎鼠体质量、平均身长等的差异均无统计学意义（P〉0．05），未见外观、内脏和骨骼出现明显异常。结论：在本实验条件下，魔芋低聚甘露糖未见明显的SD大鼠母体毒性和致畸性。OBJECTIVE： To explore the teratogenic toxicity of konjac mannan oligosaccharide, hydrolyzed by mannase, in SD rats during sensitive period of teratogenesis. METHODS ： Pregnant rats were divided randomly into four groups （12 in each group）. Three groups were fed with konjac mannan oligosaccharide solution （625, 1 250, 2 500 mg/kg） while the negative control group was given deionized water. During the sensitive period of teratogenesis （7＇h to 16th day of gestation）, pregnant rats of each group were given test sample orally once on day 20 and then sacrificed. Nidation sites, living and dead embryos, embryonic and fetal development were evaluated. Meanwhile the appearance and skeletal abnormalities were examined. RESULTS： In konjac mannan oligosaccharide treated groups, the indexes including the weight of pregnant rats and uteri including fetuses, living and dead fetus rates, body weight and length and tail of fetuses were not significantly different from negative control （P〉0.05）. No abnormality of appearance, skeleton or organ was identified in fetuses of treated groups. CONCLUTION： Under our exprimental conditions, konjac mannan oligosaccharide showed neither maternal toxicity nor teratogenicity in pregnant SD rats.

关 键 词：魔芋低聚甘露糖 SD大鼠 致畸性 致畸敏感期 

分 类 号：R994.4[医药卫生—毒理学]