Protein-assisted conjugated polymer microarray: Fabrication and sensing applications  

作　　者：Lü FengTing FENG XuLi LIU LiBing WANG Shu 

机构地区：[1]Key Laboratory of Organic Solids,Institute of Chemistry,Chinese Academy of Sciences

出　　处：《Chinese Science Bulletin》2013年第33期4039-4044,共6页

基　　金：supported by the National Natural Science Foundation of China(21203213,21021091);the National Basic Research Program of China(2011CB808400,2013CB932800)

摘　　要：A new strategy is performed to fabricate conjugated polymer microarray with the assistance of protein in this work.The water-soluble cationic conjugated polymer employed in the present work is capable of absorbing light at 510 nm,which makes it compatible with a variety of commercial microarray scanners.It is demonstrated that the protein-assisted conjugated polymer microarray exhibits higher fluorescence signal and better stability in comparison with the case without protein.The conjugated polymer microarray can be used for sensitive detection of picric acid(PA).A major advantage of our approach is its simplicity and chemical linking is not required between the conjugated polymer and microarray substrate.Considering the simplicity of the preparation of the conjugated polymer microarray,it is anticipated that novel sensing platforms will be constructed by employing this versatile method.A new strategy is performed to fabricate conjugated polymer microarray with the assistance of protein in this work. The wa- ter-soluble cationic conjugated polymer employed in the present work is capable of absorbing light at 510 nm, which makes it compatible with a variety of commercial microarray scanners. It is demonstrated that the protein-assisted conjugated polymer microarray exhibits higher fluorescence signal and better stability in comparison with the case without protein. The conjugated polymer microarray can be used for sensitive detection of picric acid （PA）. A major advantage of our approach is its simplicity and chemical linking is not required between the conjugated polymer and microarray substrate. Considering the simplicity of the preparation of the conjugated polymer microarray, it is anticipated that novel sensing platforms will be constructed by employing this versatile method.

关 键 词：共轭聚合物 微阵列 蛋白质 传感 制备 应用 聚合物芯片 荧光信号 

分 类 号：TN409[电子电信—微电子学与固体电子学]