Preparation and upconversion luminescence cell imaging of O-carboxymethyl chitosan-functionalized NaYF_4:Yb^(3+)/Tm^(3+)/Er^(3+) nanoparticles  

作　　者：LI Hao WANG LeYu 

机构地区：[1]Key Laboratory of Chemical Resource Engineering,College of Science,Beijing University of Chemical Technology

出　　处：《Chinese Science Bulletin》2013年第33期4051-4056,共6页

基　　金：supported in part by the National Natural Science Foundation of China(21275015,21075009);the National Basic Research Program of China(2011CB932403);the Program for New Century Excellent Talents in University of China(NCET-10-0213)

摘　　要：Upconversion luminescence nanoparticles(UCNPs)have shown promising applications in biomedical fields as luminescent probes because of their excellent advantages such as single excitation with multicolor emission,low autofluorescence,and deep penetration.But the biological applications of such nanomaterials are still restricted due to the unfavorable surface properties.In this work,we develop a facile one-pot hydrothermal route to obtain O-carboxymethyl chitosan(OCMC)-wrapped NaYF4:Yb3+/Tm3+/Er3+red UCNPs which have been used for targeted cell luminescence imaging directly and efficiently.The successful coating of the UCNPs by OCMC has been confirmed by Fourier-transform infrared(FTIR)spectroscopy and dynamic light scattering(DLS)studies.Transmission electron microscopy(TEM),powder X-ray diffraction(XRD),thermogravimetric analysis(TGA),and photoluminescence(PL)spectra have been used to characterize the size,composition and emission color of the samples,respectively.Due to the good biocompatibility,water-solubility,and strong UC luminescence,these hydrophilic nanocrystals will open up new avenues in further bioapplications.Upconversion luminescence nanoparticles （UCNPs） have shown promising applications in biomedical fields as luminescent probes because of their excellent advantages such as single excitation with multicolor emission, low autofluorescence, and deep penetration. But the biological applications of such nanomaterials are still restricted due to the unfavorable surface properties. In this work, we develop a facile one-pot hydrothermal route to obtain O-carboxymethyl chitosan （OCMC）-wrapped NaYF4： yb3＋/Tm3＋/Era＋ red UCNPs which have been used for targeted cell luminescence imaging directly and efficiently. The successful coating of the UCNPs by OCMC has been confirmed by Fourier-transform infrared （FTIR） spectroscopy and dynamic light scattering （DLS） studies. Transmission electron microscopy （TEM）, powder X-ray diffraction （XRD）, thermogravimetric analysis （TGA）, and photoluminescence （PL） spectra have been used to characterize the size, composition and emission color of the sam- ples, respectively. Due to the good biocompatibility, water-solubility, and strong UC luminescence, these hydrophilic nanocrystals will open up new avenues in further bioapplications.

关 键 词：O-羧甲基壳聚糖 上转换发光 细胞成像 纳米粒子 傅里叶变换红外光谱 功能化 生物相容性 透射电子显微镜 

分 类 号：TB383.1[一般工业技术—材料科学与工程]