甜瓜属种间杂交质体DNA多态性遗传分析  

Polymorphic inheritance analysis of plastid DNA in interspecific crossing of Genus Cucumis

作　　者：魏跃[1,2] 颜志明[2] 王全智[2] 薄凯亮[1] 吴志明[1] 陈劲枫[1] 

机构地区：[1]南京农业大学南方蔬菜遗传改良重点开放实验室,南京2100951 [2]江苏农林职业技术学院,江苏镇江212400

出　　处：《中国农业大学学报》2013年第6期143-147,共5页Journal of China Agricultural University

基　　金：国家自然科学基金重点项目(30830079);教育部‘111’项目(B08025);江苏省三项工程项目(SXGC(2012)373);江苏省青蓝工程项目资助

摘　　要：为研究甜瓜属种间杂交质体DNA的遗传规律,利用PCR直接测序法对甜瓜属异缘四倍体新种(Cucumis×hytivus Chen and Kirkbride.)S5自交后代及其杂交母本甜瓜属野生种(Cucumis hystrix Chakr.)和父本栽培黄瓜‘北京截头’(Cucumis sativus cv.‘Beijingjietou’)叶绿体基因Matk-trnK和rbcL-accD区域的部分DNA序列进行比较分析。结果显示在长度为2 036bp Matk-trnK区域中存在着18个多态性位点,其中有16个多态性位点的碱基子代与母本相同,只有2个位点与父本相同;在长度为945bp的rbcL-accD区域中存在着19个多态性位点,其中有18个多态性位点的碱基子代与母本相同,只有1个多态性位点与父本相同。这表明甜瓜属hystrix×sativus种间杂交中质体DNA遗传主要表现为母系遗传。To investigate the inheritance of plastid DNA in the interspecific crossing of Genus Cucumis, the DNA sequences in partial region of chloroplast Matk-trnK and rbcL-accD gene from cucumis allotetraploid （Cucumis × hytivus Chen and Kirkbride. ） S5 selfing progeny, cultivated cucumber ＇ BeijingJietou＇ （ C. sativus L. ） the paternal parents and the wild Cucumis species（ C. hystrix Chakr. ） the maternal parents, were comparatively analysed, using PCR sequencing method. Eighteen polymorphic loci were detected in 2 036 bp length Matk-tmK gene region, sixteen polymorphic loci in allotetroploid as the its maternal parents,two as its paternal parents. Nineteen polymorphic loci were detected in 945 bp length rbcL-accD gene region, eighteen polymorphic loci in allotetraploid as its maternal parents, only one as its paternal parents. The result demonstrated that plastid genomes was maternal inheritance pattern in hystrix x sativusin species crosssing among Genus Cucumis.

关 键 词：甜瓜 质体 多态性位点 母系遗传 

分 类 号：S642.2[农业科学—蔬菜学]