34种铁兰属植物亲缘关系的SRAP分析  

Genetic relationship of 34 Tillandsia plants using SRAP marker

作　　者：宿静[1] 李玉萍[2] 汤庚国[3] 

机构地区：[1]东营市技师学院,山东东营257091 [2]金陵科技学院园艺学院,江苏南京210038 [3]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2013年第5期153-156,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省镇江市科技计划项目(NY2007044)

摘　　要：利用SRAP分子标记对34种铁兰属植物的亲缘关系进行分析。挑选了10对特异性引物，共扩增出402个位点，多态性位点376个，多态性百分率94％，相似系数0．1343—0．9556。聚类分析发现，34份铁兰属植物材料在相似系数为0．78时可划分为4个类群。分布地域是影响聚类结果的重要影响因子，实验证明Tillandsiajuncea与rsubgen．Anoplophytum亚属有较近的亲缘关系。In this paper, SRAP was used to assess the genetic relationship of 34 Tillandsia varieties. Ten pairs of prim- ers were selected. 402 bands were amplified, of which 376 bands （94%）were polymorphic, and the Nei＇s genetic simi- larity coefficients ranged from 0. 134 3 -0. 955 6. According to the UPGMA method,we made the cluster analysis by u- sing the NTSYS software,3g Tillandsia plants could be divided into four groups at the similarity coefficient of 0.78. The geographical distribution was a principal factor for the cluster result. The experiment indicated that TiUandsia juncea and T. subgen. Anoplophytum had close relationship.

关 键 词：铁兰属 亲缘关系 SRAP 

分 类 号：Q948[生物学—植物学]