利用两种活体荧光技术分析北极微藻对光照变化的响应  

STUDIES ON RESPONSE OF ARCTIC MICROALGAE TO CHANGE OF IRRADIANCE,APPLYING TWO KINDS OF FLUORESCENT TECHNIQUES

作　　者：王肖颖[1,2] 何剑锋[1] 光应芝[1] 李娟英[2] 张芳[1] 

机构地区：[1]国家海洋局极地科学重点实验室,中国极地研究中心,上海200136 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《极地研究》2013年第3期257-267,共11页Chinese Journal of Polar Research

基　　金：国家自然科学基金(41076130;41206189)资助

摘　　要：采用调制叶绿素荧光技术(脉冲-振幅-调制:Pulse-Amplitude-Modulation,简称为PAM技术)和荧光光谱两种技术研究不同光照下的两种北极微藻(北极异帽藻(Heterocapsa arctica)和红胞藻(Rhodomonas sp.)的荧光特性。研究发现:(1)两种藻在三个不同光照水平下(5 000 lux、15 000 lux、25 000 lux)光合生理变化特征相似,随着光照强度增加,光量子产量越低,光合效率越低,在15 000 lux和25 000 lux两个较强光照条件下,最大光合电子传递速率(rETRmax)没有提高,且捕光能力下降,在25 000 lux光照强度下,均处于胁迫状态,但对强光的耐受能力增强;(2)两种藻在15 000 lux条件下Chl a荧光强度最高,25 000 lux条件下的Chl a荧光强度都显著降低,受到不同程度的光损伤,类胡萝卜素和多甲藻素是维持北极异帽藻15 000 lux下种群生存的有效色素,藻胆素和Chl c则分别为维持红胞藻5 000 lux和15 000 lux条件下的种群的生存起了积极作用。This work investigated fluorescence characteristics of two Arctic algae species （ Heterocapsa arctica and Rhodo- monas sp. ） via two types of in vivo fluorescence techniques, pulse-amplitude modulation （PAM） and fluorescence spectrum. The main results are ： （ 1 ） The photosynthetic physiology of two kinds of algae were similar under three levels of irradiance （5 000, 15 000 and 25 000 lux）. For stronger irradiance, the value of FJFm and photosyn- thetic efficiency were higher; under 25 000 lux, the algae species were in a stress state, but their endurance capac- ity was enhanced. （2） Fluorescence intensity of Chlorophyll a of the two algae species was highest under 15 000 lux. That intensity decreased for 15 000 lux. Carotenoids and peridinins were the effective pigments for survival of Heterocapsa arctica under 15 000 lux; phycobilin and Chlorophyll c had a positive effect on maintaining Rhodo- monas sp. under 5 000 lux and 15 000 lux.

关 键 词：北极微藻 光照 调制叶绿素荧光 荧光光谱 

分 类 号：Q948.8[生物学—植物学]