脊椎动物趋化因子受体CXCR基因分化研究  

Diversification Study of CXC Chemokine Receptor in Vertebrate

作　　者：庹锐[1] 许巧情[1] 万晶[1] 

机构地区：[1]长江大学动物科学学院,中国湖北荆州434020

出　　处：《生命科学研究》2013年第5期377-386,共10页Life Science Research

基　　金：国家自然科学基金资助项目(31101928);长江大学优秀青年教师科研基金资助项目(cyq201305)

摘　　要：为了探讨脊椎动物CXCR(CXC chemokine receptor)在基因组上进化和分化的规律,采用生物信息学软件绘制了脊椎动物7个CXCR的基因结构图,分析了它们的系统进化关系,研究这些受体在不同物种基因同源性.结果表明,脊椎动物CXCR 7个成员在进化上发生了不同程度地分化.人、鼠、蜥蜴CXCR1和CXCR2在同一条染色体上,蛋白相似率很高,在进化树上混杂聚集在一起,形成CXCR1/2;而硬骨鱼类CXCR1和CXCR2发生了分化,形成了单独的CXCR1和CXCR2.鱼类CXCR3分化为3个基因,其中CXCR3b1、CXCR3b2与其他脊椎动物CXCR3同源性较高,聚集在一起,而CXCR3a分化较大.脊椎动物CXCR5和CXCR6基因较保守,基因同源性高.CXCR4和CXCR7在哺乳类、鸟类、爬行类和两栖类均仅一个基因,但在硬骨鱼类中它们各自分化为2个基因.CXCR4和CXCR7位于同一条染色体上.鱼类CXCR4a和CXCR7a与其他脊椎动物CXCR4和CXCR7基因同源性较高.而CXCR4b和CXCR7b这两个基因无论是从基因结构还是基因同源性上都发生了一定程度的分化.In order to explore the evolution and diversification rule of CXCRs in vertebrate genome, genomic structure was drawn using biological information software, then phylogenetic relationship and gene synteny were analyzed. The results showed that CXCR1-7 have undergone genetic differentiation in different degree. In human, mouse and lizard, CXCR1 and CXCR2 located in the same chromosome, with a high identity between each other, and clustered to a mixed group （CXCR1/2） in phylogenetic tree. However, CXCR1 and CXCR2 separated each other and clustered to CXCR1 group and CXCR2 group in bony fish. Teleosts CXCR3 has evolved to other vertebrate CXCR3 three genes, in which CXCR3bl and CXCR3b2 had a relative high identity with and they were clustered together. But CXCR3a diverged a lot. CXCR5 and CXCR6 were conservative and they have high identity in different species. CXCR4 and CXCR7 only have one gene in mammal, birds, reptiles, amphibian but they have evolved into two genes in teleosts. Furthermore, CXCR4 and CXCR7 located in the same ehromosome. CXCR4b and CXCR7b in teleosts have undergone great differentiation not only in genomic structure but also gene synteny.

关 键 词：CXCR 基因结构 基因同源性 基因分化 

分 类 号：Q71[生物学—分子生物学]