高效液相色谱法测定芪白平肺胶囊中人参皂苷R_(g1)与Rb_1含量  

High Performance Liquid Chromatography Method for Determination of Ginsenosides R_(g1) and Rb_1 in Qibai Pingfei Capsules

作　　者：王芳[1] 王斌[1] 张贺[1] 葛平[1] 李泽庚[1] 孟楣[1,2] 

机构地区：[1]安徽中医药大学研究生部,安徽合肥230038 [2]安徽中医药大学第一附属医院 国家中医药管理局中药制剂三级实验室,安徽合肥230031

出　　处：《安徽中医学院学报》2013年第5期72-75,共4页Journal of Anhui Traditional Chinese Medical College

基　　金：安徽省高校省级自然科学研究项目(KJ2011Z218);安徽省2012年中医发展省级专项(皖中医药[2012]10号)

摘　　要：目的采用高效液相色谱法测定芪白平肺胶囊中人参皂苷Rg1与Rb1的含量，为其质量控制提供依据。方法采用WelchMaterialIncC18色谱柱（4．6mm×250mm，5μm）。其流动相：水-乙腈；梯度洗脱（0～10min，81：19；11～35min，81：19—71：29；36～50min，71：29—50：50）；流速：1．2ml／min；检测波长：203nm；柱温：30℃。结果人参皂苷Rg1、Rb1在0．16～2．40μg范围内与其峰面积呈线性关系，r=0．9999（n=8）；人参皂苷Rg1、Rb1的平均加样回收率分别为102．31％、98．55%（n=9）；芪白平肺胶囊中人参皂苷Rg1、Rb1的3批样品测定结果，人参皂苷Rg1平均含量分别为0．332、0．326、0．336mg／g，人参皂苷Rb1平均含量分别为0．496、0．509、0．511mg／g。结论所建立的高效液相色谱方法简便、灵敏度高、重现性好，可用于芪白平肺胶囊中人参皂苷的含量测定。Objective To establish a method for simultaneous determination of ginsenosides Rg1 and Rb1 in Qibai Pingfei Capsules by high-performance liquid chromatography （HPLC） and to provide a basis for the quality control of Qibai Pingfei Capsules. Methods HPLC was performed on a Welch Material Inc C18 column （4.6 mm × 250 mm, 5 μm） with a mobile phase of wateacetonitrile for gradient elution at a flow rate of 1..2 ml/min, a detection wavelength of 203 nm, and a column temperature of 30 ℃. The water/acetonitrile ratio was 81：19 at 0-10 min, ranged from 81：19 to 71：29 at 11-35 min, and ranged from 71：29 to 50：50 at 36-50 min. Results Ginsenosides Rg, and Rb1 showed a linear relationship between concentration and peak area within 0.16-2.40 μg （r= 0. 999 9, n=8）; the average recovery rates of ginsenosides Rg1 and Rb1 were 102.31% and 98.55%, respectively （n=9）. In the three batches of Qibai Pingfei Capsule samples, the average contents of ginsenoside Rg1 were 0. 332, 0. 326, and 0. 336 mg/g, respectively, and the average contents of ginsenoside Rb1 were 0. 496, 0. 509, and 0. 511 mg/g, respectively. Conclusion This HPLC method is simple, with high sensitivity and good repeatability. It can be used for the determination of ginsenosides ig1 and Rb1 in Qibai Pingfei Capsules.

关 键 词：高效液相色谱法 梯度洗脱 芪白平肺胶囊 人参皂苷 含量测定 

分 类 号：R927.2[医药卫生—药学]