大鼠Sertoli细胞对骨髓间充质干细胞体外诱导分化的影响  

The Effects of Sertoli Cells on the Induced Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

作　　者：应涵汝[1] 蒋黎华[1] 王韦仑[1] 李伟毅[1] 

机构地区：[1]上海交通大学医学院免疫学与微生物学系,上海市免疫学研究所,上海市200025

出　　处：《组织工程与重建外科杂志》2013年第5期260-262,共3页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：上海市卫生局科研计划(2009208);上海市重点学科建设项目上海市免疫学研究所基金

摘　　要：目的探索Sertoli细胞(SCs)对骨髓间充质干细胞(BMSCs)成脂、成骨和成神经诱导分化的影响。方法密度梯度离心法分离BMSCs,二步酶消化法分离SCs;对第3、4代BMSCs进行成脂、成骨及成神经诱导,并与SCs共培养,观察其形态学变化。结果油红O染色显示BMSCs成脂诱导后,胞浆出现折光性强的脂滴,加入SCs后,脂滴明显减少,染色不明显;茜素红S染色显示BMSCs成骨诱导后可显示钙结节,加入SCs后,钙结节更多,结节内红染更明显;成神经诱导在加入SCs前后没有明显差异。结论 SCs可促进BMSCs成骨分化,而抑制其成脂分化,对成神经分化则没有明显影响。Objective To explore the effects of Sertoli cells on the induced adipogenic, osteogenic and neuroblastic differentiation of bone marrow mesenchymal stem cells （BMSCs）. Methods BMSCs were isolated and purified with the density gradient centrifugation method, Sertoli cells were isolated with two-step enzyme digestion;The adipogenic, osteogenic and neuroblastic differentiation of P3/P4 BMSCs were induced. Sertoli cells were co-cultured with BMSCs and then the morphological change of BMSCs were observed. Results After adipogenic induction, the lipid droplets that have strong refraction in the cytoplasm were observed by oil red O staining. The intracellular lipid droplets shrunk and the orange stained lipid droplets were hard to observe after adding Sertoli cells; After osteogenic induction, calcium nodules were shown by alizarin red S staining. More clearer calcium nodules were observed and the red-staining was stronger after adding Sertoli cells. No morphological change was observed on BMSCs before and after adding Sertoli cells. Conclusion Sertoli cells could promote BMSCs’ osteogenic differentiation, inhibit their adipogenic differentiation and have no effect on their neuroblastic differentiation.

关 键 词：骨髓间充质干细胞 SERTOLI细胞 诱导分化 

分 类 号：Q813.11[生物学—生物工程]