基因枪法获得转CBF4基因蒙农杂种冰草的研究  

Studies on Transformation of Agropyron cristatum×A.desertorum cv.Mengnong with CBF4by Particle Bombardment

作　　者：徐春波[1] 王勇[2] 赵来喜[1] 赵海霞[1] 米福贵[2] 

机构地区：[1]中国农业科学院草原研究所,内蒙古呼和浩特010010 [2]内蒙古农业大学生态环境学院,内蒙古呼和浩特010018

出　　处：《中国草地学报》2013年第5期24-28,共5页Chinese Journal of Grassland

基　　金："十二五"国家科技支撑计划项目(2012BAD13B07);中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)项目(1610332012207);内蒙古自然科学基金项目(2011MS0402)资助

摘　　要：为快速培育出优良的冰草品种,以蒙农杂种冰草(Agropyron cristatum×A.desertorumcv.Mengnong)幼穗为材料,以拟南芥外源脱水应答转录因子CBF4基因为目的基因,利用基因枪轰击蒙农杂种冰草幼穗诱导愈伤组织,经筛选后获得了抗性转化植株,进一步经PCR和RT-PCR检测得到了670bp左右的电泳条带,表明CBF4基因已在蒙农杂种冰草中得到表达。In order to speed up the process of wheatgrass germplasm improvement and cultivate more excellent varieties, Agropyron cristatum X A. desertorurn cv. Mengnong＇s young spikes were used as ma- terials, CBF4 was used as target gene and the spike callus were bombarded with gene gun. The regenera- ted plants were screened and tested by PCR and RT-PCR. The electrophoresis band about 670bp showed that the CBF4 gene was expressed in Agropyron cristaturn X A. desertorurn cv. Mengnong. The studies laid a good foundation for breeding new drought-resistance wheatgrass varieties.

关 键 词：蒙农杂种冰草 CBF4基因 遗传转化 抗旱性 

分 类 号：S503.53[农业科学—作物学]