用小RNA深度测序鉴定广西冬种马铃薯病毒  

Identification of Viruses from Potato Planted in Winter in Guangxi by Small RNA Deep Sequencing

作　　者：宋静静[1] 蒙姣荣[1,2] 邹承武[3] 李璞[3] 王志强[2] 陈保善[2,3] 

机构地区：[1]广西大学农学院,南宁530004 [2]广西大学亚热带农业生物资源保护与利用国家重点实验室,南宁530004 [3]广西大学生命科学与技术学院,南宁530004

出　　处：《中国农业科学》2013年第19期4075-4081,共7页Scientia Agricultura Sinica

基　　金：国家重点基础研究"973"计划项目(2006CB101900)

摘　　要：【目的】对广西冬种马铃薯病毒进行鉴定,为无病毒种薯选择和大田防治提供依据。【方法】2012年在马铃薯主要产区采集具有明显病毒病症状的样品,在血清学ELISA检测的基础上,进一步用小RNA深度测序对按症状分类的样品进行混合样本的病毒种类鉴定,再用RT-PCR方法对分组混合样本进行验证。【结果】在109个样本中检测到马铃薯卷叶病毒(Potato leafroll virus,PLRV)、马铃薯A病毒(Potato virus A,PVA)、马铃薯H病毒(Potato virus H,PVH)、马铃薯M病毒(Potato virus M,PVM)、马铃薯S病毒(Potato virus S,PVS)、马铃薯Y病毒(Potato virus Y,PVY)和马铃薯纺锤块茎类病毒(Potato spindle tuber viroid,PSTVd),同时发现PVS有丰富的株系分化。【结论】近年广西冬种马铃薯病毒种类增多,病症多样,亟需加强种薯管理,培育无毒健康种薯。【Objective】The objective of this study is to identify viruses infecting potato planted in winter in Guangxi for establishing a future healthy potato seed program and better disease control in the field. 【Method】One hundred and nine samples with viral disease-like symptoms were collected from potato plants in the fields in 2012 and subject to initial ELISA screening before they were pooled and analyzed by small RNA deep sequencing. Viruses identified by small RNA sequencing were validated by virus-specific RT-PCR from selected groups of samples.【Result】Potato leafroll virus （PLRV）, Potato virus A （PVA）, Potato virus H （PVH）, Potato virus M （PVM）, Potato virus S （PVS）, Potato virus Y （PVY）, and Potato spindle tuber viroid （PSTVd） were detected in the samples and PVS was found to have a rich genetic diversity.【Conclusion】Potato planted in winter in Guangxi were infected with many viruses including two viruses and a viroid that had not been reported in the region before. Thus, a virus-free healthy potato seed program is needed in urgency.

关 键 词：小RNA高通量测序 广西 冬种马铃薯 病毒病害 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]