基因调控型质粒串联表达猪α、γ干扰素及其体外抗猪繁殖与呼吸综合征病毒活性研究  

Coexpression of porcine alpha and gamma interferons by gene regulatory plasmid and its anti-PRRSV activity in vitro

作　　者：周顺[1] 刘纪玉[1,2] 赵红波[2] 张怡轩[1,2] 郭立辉[2] 吴家强[1,2] 时建立[2] 任素芳[2] 王金宝[1,2] 杜以军[1,2] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]山东省畜禽疫病防治与繁育重点实验室/山东省农业科学院畜牧兽医研究所,山东济南250100

出　　处：《畜牧与兽医》2013年第10期16-20,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31100119;31170146;31370189);农业科技成果转化资金项目(2011GB2C600011);公益性行业(农业)科研专项(201303046);山东省优秀中青年科学家科研奖励基金(BS2012NY012);中国博士后科学基金(2012M521341)

摘　　要：利用兔β-Globin Intron II基因调控序列,改造真核表达载体pVAX1,构建基因调控型质粒,将猪α干扰素(pIFN-α)和γ干扰素(pIFN-γ)进行串联表达。结果表明,重组质粒pMVAX1-pIFN-α/γ表达的IFN活性相比pVAX1-pIFN-α提高了100倍,比pVAX1-pIFN-γ提高了1 000倍。体外抗病毒活性表明,pIFN-α/γ的量为100 U(稀释度1∶106)以上时,对100 TCID50的高致病性猪繁殖与呼吸综合征病毒(PRRSV)具有明显的抑制效果,说明串联表达的pIFN-α和pIFN-γ具有高效抗PRRSV效果。本研究将为PRRS的预防治疗提供新的思路和方法。In this study, the rabbit β-Globin Intron II gene regulatory sequence was used to modify the eukaryotic expression vector pVAX1 and the gene regulatory plasmid was constructed. Then porcine alpha and gamma interferons （pIFN-ot and pIFN-γ） were coexpressed by the gene regulatory plasmid. The results showed that compared to pVAX1-pIFN-α and pVAX1-pIFN-α/γ, the activity of expressed IFN from the recombinant plasmid pMVAXI-pIFN-α/γ increased 100 and 1000 times, respectively. At 100U pIFN-α/γ （dilution 1 ： 106） or above, highly pathogenic porcine reproductive and respiratory syndrome virus （PRRSV） could be obviously inhibited. It suggested that pIFN-α and pIFN-γ co-expressed by the gene regulatory plasmid had high activity against PRRSV. This study will provide new ideas and methods for prevention and treatment of PRRS.

关 键 词：猪Α干扰素 猪Γ干扰素 猪繁殖与呼吸综合征病毒 抗病毒活性 

分 类 号：S852.6[农业科学—基础兽医学]