原花青素及其组合物对泡沫细胞形成的影响  

Effects of Proanthocyanidins and its composition on the formation of foam cells

作　　者：苗智如[1] 廖丽娜[2] 张敏敏[2] 蒋国军[3] 

机构地区：[1]杭州市萧山区第一人民医院药剂科,311201 [2]解放军第二军医大学药学院 [3]浙江萧山医院

出　　处：《浙江医学》2013年第18期1644-1647,共4页Zhejiang Medical Journal

基　　金：浙江省药学会医院药学科研基金项目(2010ZYY15);杭州市卫生局卫生科技计划重点项目(2011Z019)

摘　　要：目的观察原花青素及其与抗炎活性成分(积雪草苷、姜黄素、茶多酚)的组合物对氧化低密度脂蛋白(ox-LDL)刺激巨噬细胞形成泡沫细胞过程中炎症细胞因子和细胞内胆固醇含量的变化。方法以体外培养的小鼠单核巨噬细胞系RAW264.7细胞为材料和ox-LDL刺激RAW264.7细胞24h形成泡沫细胞为模型,RT-PCR方法检测细胞因子IL-1β和TNF-αmRNA水平,试剂盒测定细胞内游离胆固醇和总胆固醇的含量。结果原花青素及其与积雪草苷、姜黄素、茶多酚组合物可明显降低泡沫细胞内的胆固醇酯与总胆固醇的比值,抑制泡沫细胞的形成,显著抑制泡沫细胞的IL-1β和TNF-αmRNA表达,其中原花青素与姜黄素的组合物作用最强。结论原花青素组合物通过降低细胞内胆固醇酯的含量,减轻细胞泡沫化程度和减轻细胞的炎症反应起到抗动脉粥样硬化的作用。Objective To study the effect of Proanthocyanidins and its composition with Anti- inflammatory active ingredi-ents such as Asiaticoside, Curcumin or tea polyphenols on cytokine levels and intracellular cholesterol during foam cellformation induced by oxidized low density lipoprotein （ox- LDL）. Methods Mouse monocyte- macrophage cell line RAW264.7 cells were cultured in vitro and the cells were stimulated by ox- LDL for 24h to transform foam cells. IL- 1βmRNA and TNF- α mRNA was evaluated by real time PCR. An enzymatic colorimetric assay was used to determine intracellular cholesterol. Results Proantho-cyanidins and its composition with Asiaticoside, Curcumin or tea polyphenols can significantly reduced the cholesterol esters and the ratio of total cholesterol in the foam cells, inhibite foam cells formation and expression of IL- 1βmRNA and TNF- α mRNA . The composition of Proanthocyanidins and curcumin had the strongest effect. Conclusion Proanthocyanidins and its composi-tion play a role of anti- atherosclerosis by reducing intracellular cholesterol ester content, foam cells formation and the inflamma-tory response.

关 键 词：原花青素 RAW264 7细胞 泡沫细胞 细胞因子 

分 类 号：R285.5[医药卫生—中药学]