Effect of Huanglianjiedu Tang on fever in rats induced by 2, 4-dinitrophenol  

作　　者：Shumin Liu Na Wang Pingping Chen Xuzhao Li Changfeng Liu 

机构地区：[1]Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine

出　　处：《Journal of Traditional Chinese Medicine》2013年第4期492-499,共8页中医杂志（英文版）

基　　金：Supported by the National Basic Research Program of China(No.2007CB512608)

摘　　要：OBJECTIVE: To study metabolic characteristics of fever in rats induced by 2, 4-dinitrophenol (DNP) and the effect of Huanglianjiedu Tang (HLJDT) on the fever. METHODS: The urine samples were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) at the positive ion mode scanning, and experimental data were analyzed by the principal component analysis. RESULTS: Eight potential biomarkers indicating the occurrence and evolvement of fever were determined according to ions in urine samples. Five of them were found increased, while the other three decreased. After HLJDD intervention, the increased five were reduced significantly in high dose group, compared with model group, while the decreased three had no obvious change. Five of the eight biomakers were identified with formyl-5-hy-droxykynurenamine, gentisic acid, aminoadipic acid, phenylacetic acid, L-phenylalanyl-L-hydroxyproline on the basis of MS/MS.These biomarkers are associated with the metabolism of 5-hydroxytryptamine, tyrosine, lysine, phenylalanine and collagen protein, respectively. CONCLUSION: HLJDT had significant effect on DNP-induced fever in rats. The effect was performed possibly by acting on 5-hydroxytryptamine in hypothalamus and some amino acid metabolism. These results suggested that HLJDT relieved fever by acting on multi-targets.OBJECTIVE： To study metabolic characteristics of fever in rats induced by 2, 4-dinitrophenol （DNP） and the effect of Huanglianjiedu Tang （HLJDT） on the fever. METHODS： The urine samples were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry （UPLC/ESI-Q-TOF-MS） at the positive ion mode scanning, and experimental data were analyzed by the principal component analysis. RESULTS： Eight potential biomarkers indicating the occurrence and evolvement of fever were determined according to ions in urine samples. Five of them were found increased, while the other three decreased. After HLJDD intervention, the increased five were reduced significantly in high dose group, compared with model group, while the decreased three had no obvious change. Five of the eight biomakers were identified with formyl-5-hy-droxykynurenamine, gentisic acid, aminoadipic acid, phenylacetic acid, L-phenylalanyl-L-hydroxyproline on the basis of MS/MS.These biomarkers are associated with the metabolism of 5-hydroxytryptamine, tyrosine, lysine, phenylalanine and collagen protein, respectively. CONCLUSION： HLJDT had significant effect on DNP-induced fever in rats. The effect was performed possibly by acting on 5-hydroxytryptamine in hypothalamus and some amino acid metabolism. These results suggested that HLJDT relieved fever by acting on multi-targets.

关 键 词：2 4-dinitrophenol Heat syndrome Chromatography liquid Metabolism HuanglianjieduTang 

分 类 号：R965[医药卫生—药理学]