两种方法检测牛地方流行性白血病的比较  

Comparison of indirect ELISA and AGID for Detecting EBLV Antibody in the Serum Samples Dairy Cattle

作　　者：王作佳[1] 赵祥平[1] 王乃福[1] 张永年[1] 王玉玲[1] 刘亚峰[1] 董志珍[1] 

机构地区：[1]天津出入境检验检疫局,天津300461

出　　处：《中国动物检疫》2013年第10期74-76,共3页China Animal Health Inspection

基　　金：天津检验检疫科技技术项目(编号:TK006-2011)

摘　　要：目的分析ELISA方法和AGID方法检测奶牛地方流行性白血病的一致性。方法应用酶联免疫吸附试验(ELISA)和琼脂凝胶免疫扩散试验(AGID)两种方法,平行检测来自乌拉圭的3998份奶牛血清中的牛白血病病毒抗体。结果 ELISA和AGID对牛地方流行性白血病病毒抗体的阳性符合率、阴性符合率及总符合率分别达到100%(18/18)、99.42%(3975/3998)、99.87%(3993/3998),表明两种方法的符合率较高,均可用于奶牛地方流行性白血病的诊断和血清学筛查,其中ELISA方法阳性检出率高于AGID方法。Objective to analyze the consistency of ELISA and AGID for detecting BLV antibody. Method The 3998 Se-rum samples of cows imported from Uruguay were collected and tested for the antibodies against BLV using ELISA and AGID. Result The results showed that the positive coincidence rate of the two methods was 100%（18/18）,the nega-tive coincidence was 99.42%（3975/3998） and the total coincidence rate was 99.87%（3993/3998）. Conclusion Both ELISA and AGID could be applied in diagnosis and serological screening of EBL,and the ELISA positive rate was higher than that of AGID method.

关 键 词：酶联免疫吸附试验(ELISA) 琼脂凝胶免疫扩散试验(AGID) 牛白血病病毒(BLV) 

分 类 号：S858.23[农业科学—临床兽医学]