新疆地区规模化奶牛场牛支原体流行病学调查  

INVESTIGATION INTO MYCOPLASMA BOVIS INFECTION ON LARGE-SCALE DAIRY FARMS IN XINJIANG

作　　者：李岩[1] 姚永进[2] 范伟兴[3] 剡文亮[1] 石刚[2] 剡根强[2] 

机构地区：[1]新疆生产建设兵团畜牧兽医工作总站,乌鲁木齐830063 [2]石河子大学动物科技学院,石河子832003 [3]中国动物卫生与流行病学中心,青岛266032

出　　处：《中国动物传染病学报》2013年第5期68-71,共4页Chinese Journal of Animal Infectious Diseases

基　　金：国家科技支撑计划课题(2012BAD43B02);兵团农业科技攻关(2013BA004);石河子市科技计划(2011NY09)

摘　　要：为了调查新疆地区规模化奶牛场牛支原体的感染情况,采用牛支原体间接ELISA和特异性PCR检测牛血清中的牛支原体抗体及病料中牛支原体核酸,共检测9个地区15个规模化奶牛场437份血清,肺脏、关节液及鼻腔黏液44份。结果显示,血清抗体阳性率为76.43%(334/437),其中脐带血抗体阳性率为40.00%(4/10);病料阳性率为40.91%(18/44)。检测结果表明,新疆地区大部分奶牛场存在牛支原体感染,部分奶牛场发生牛支原体肺炎及关节炎病例,并存在垂直传播的风险。牛支原体感染可能成为危害新疆规模化奶牛场犊牛健康的主要疫病之一。The present study was undertaken to investigate into Mycoplasma bovis infection on large-scale dairy farms in Xinjiang. Total 427 serum samples, 10 umbilical cord blood samples and 44 diseased tissue samples were collected from 15 large-scale dairy farms located in 9 agriculture and animal husbandry divisions. These samples were examined using indirect ELISA and PCR to determine the presence of Mycoplasma boris antibodies and/or nucleic acid materials. The positive rates in serum, umbilical cord blood, diseased tissue samples were determined to be 78.45% （335/427）, 40.00% （4/10） and 40.91% （18/44）, respectively. These results indicated the prevalence of Mycoplasma boris infection on dairy farms. Some dairy cows suffered from pneumonia and arthritis caused by Mycoplasma bovis and vertical transmission might play a role. These findings provided suggesfed that Mycoplasma bovis infection was prevalent in dairy cow population and might make a major factor for economic loss of dairy farmers in Xinjiang.

关 键 词：奶牛 牛支原体 PCR 感染 间接ELISA 

分 类 号：S852.62[农业科学—基础兽医学]