转基因大豆A5547-127品系成分LAMP快速检测方法的建立  

Development of Loop-mediated Isothermal Amplification(LAMP)Method for Rapid Detection of Transgenic Soybean A5547-127 Component

作　　者：蔡颖[1] 周广彪[1] 刘津[2] 高东微[2] 凌莉[2] 刘静宇[2] 易敏英[2] 吴少云[3] 李志勇[2] 

机构地区：[1]汕头出入境检验检疫局技术中心,广东汕头515031 [2]广东检验检疫技术中心,广东广州510623 [3]华南理工大学生物科学与工程学院,广东广州510641

出　　处：《食品研究与开发》2013年第17期69-73,共5页Food Research and Development

基　　金：出入境检验检疫行业标准制定计划项目:出口食品中转基因成分环介导等温扩增(LAMP)检测方法(2011B286k);广东省科技计划项目(2009B0627):食品转基因成分快速检验能力建设(粤科函财字[2009]627号);广东检验检疫局科技计划项目:主要转基因食品的快速筛查技术及装置研究(2009GDK45)

摘　　要：根据转基因大豆A5547-127品系外源插入片段与植物基因组序列设计品系特异性引物,筛选最佳引物并对反应体系和反应条件进行优化,最终建立了转基因大豆A5547-127品系成分特异性LAMP的快速检测方法。对该方法进行了特异性、灵敏度和稳定性测试。结果表明:该方法能快速、特异性检测出转基因大豆A5547-127品系成分,定性检测低限为0.5%,稳定性和特异性均能达到100%。According to exogenous insert sequences connection with the plant genome sequences of transgenic soybean A5547-127, several event specific primer sets were designed. The optimum was selected from these candidates, then the reaction system and reaction conditions were optimized. Finally a loop-mediated isothermal amplification （LAMP） method for rapidly detecting transgenic soybean A5547-127 component was developed. The performances, including specificity, sensitivity and stability were tested with this method. The results showed that the method can specifically detect transgenic soybean A5547-127 component rapidly, with limit of detection （LOD） as low as 0.5 %, stability and specificity both of 100 %.

关 键 词：环介导等温扩增 品系特异性 转基因大豆A5547-127品系 检测 

分 类 号：S565.1[农业科学—作物学]