高效液相色谱法测定复方甘草搽剂中甘草酸含量  

Determination of Glycyrrhizic Acid in Compound Glycyrrhiza Liniment by HPLC

作　　者：曹阳虎 郑江萍[2] 黄良永[2] 

机构地区：[1]湖北江汉油田总医院,湖北潜江433124 [2]湖北医药学院附属太和医院药学部,湖北十堰442000

出　　处：《中国药业》2013年第20期42-43,共2页China Pharmaceuticals

摘　　要：目的建立测定复方甘草搽剂中甘草酸含量的高效液相色谱法。方法采用CosmosilC．。柱（美国Waters公司，250mm×4．6mm，5μm），甲醇-0．2mol／L醋酸铵一冰醋酸（65：35：1）为流动相，流速1mL／min，检测波长241nm，柱温30℃。结果甘草酸峰能与其他杂质峰完全分离，甘草酸质量浓度在10．40～l039．60μg／mL范围内与峰面积有良好的线性关系，平均回收率为99．63％，RSD为0．24％。结论所用方法可测定复方甘草搽剂中甘草酸的含量，准确度高，重复性好，可作为制剂的质量控制方法。Objective To establish a HPLC method for the determination of glycyrrhizic acid in Compound Glycyrrhiza Lini- ment. Methods The Cosmosil C18 column （250 mm-4.6 mm, 5 p,m） was adopted with the mobile phase of methanol -0.2 mol/L ammonium acetate-glacial acetic acid（65：35：1）, the flow rate was 1 mL/min, the detection wavelength was 241 nm and the col- umn temperature was 30 %. Results The peak of glycyrrhizic acid could be completely separated from the other impurities peaks without interference; the linear range of glycyrrhizic acid was 10.40- 1 039.60 μg/mL, the average recovery rate was 99.63% （n = 6）, RSD = 0, 24%. Conclusion This method is highly accurate and better reproducible for detecting the glycyrrhizic acid content in Com- pound Glycyrrhiza Liniment, and can be used as the quality control method of this preparation.

关 键 词：高效液相色谱法 甘草酸 复方甘草搽剂 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]