p53基因敲除小鼠生长发育和繁殖性能的调查  

A Survey of Growing Development and Reproductive Performance of p53 Knockout Mice

作　　者：徐萌[1] 柴梦音[1] 绳波[1] 陈德喜[1] 

机构地区：[1]首都医科大学附属北京佑安医院,北京100069

出　　处：《实验动物科学》2013年第4期6-8,共3页Laboratory Animal Science

基　　金：国家自然科学基金项目(ASPP2和ASPP2在肝组织中表达失衡与肝癌发生的机制)(No.81272266)

摘　　要：目的观察p53基因敲除小鼠与BALB/c小鼠在生长发育和繁殖性能方面的差异。方法 p53基因敲除小鼠和BALB/c小鼠各34只,雌雄各半,观察其2周龄至12周龄的体质量和体长,并随机选取30笼一雌一雄所产第2胎仔鼠进行幼仔个数的统计。结果在生长发育方面,3周龄时p53基因敲除小鼠与BALB/c小鼠体质量无明显差异,P=0.846>0.05;6周龄时p53基因敲除小鼠体质量明显低于BALB/c小鼠,P=0.000<0.05;体长在3周龄和6周龄时p53基因敲除小鼠体长明显低于BALB/c小鼠,P=0.000<0.05。在繁殖性能方面,p53基因敲除小鼠同样明显低于BALB/c小鼠,P=0.000<0.05。结论初步研究了p53基因敲除对小鼠的繁殖与发育的影响,与BALB/c小鼠比较均有显著差异。Objective To investigate the differences in growing development and reproductive performance between p53 knockout mice and BALB/c mice.Method 34 p53 knockout mice with half males and half females and 34 BALB/c mice （half male and female） were adopted in the trial,and their body weight and body length were measured from 2 to 12 weeks of age.Litter sizes at the second parity from 30 pairs of mice were checked.Result The difference in body weight between 3-week old p53 knockout mice and BALB/c mice was not significant （P ＞ 0.05）,and body weight of 6-week old p53 knockout mice was significantly lower than that of 6-week old BALB/c mice （P ＜0.05）.Body length of 3-week and 6-week old p53 knockout mice was significantly lower than that of 3-week and 6-week old BALB/c mice （P ＜ 0.05）.Reproductive performance of p53 knockout mice was significantly lower than that of BALB/c mice （P ＜ 0.05）.Conclusion p53 gene knockout could exert significant effect on the growing development and reproductive performance of the mice.

关 键 词：p53基因敲除小鼠 生长发育 繁殖性能 

分 类 号：Q95-33[生物学—动物学]