大鼠多次采血常用的三种方法探讨  

Exploration on Three Common Methods of Multiple-drawblood in Rat

作　　者：王明丽[1] 张晶[1] 王耀辉[1] 李淑莲[1] 马远方[1] 

机构地区：[1]河南大学医学院细胞与分子免疫学重点实验室,开封475004

出　　处：《实验动物科学》2013年第4期44-45,43,共3页Laboratory Animal Science

基　　金：国家自然科学基金(No.30972687)

摘　　要：目的探讨大鼠在1天内或者短时间内需要多次采血常用的三种方法,提高大鼠实验的成功率。方法采用90只雄性Wistar大鼠,分别用眼眶后静脉采血法、颈静脉采血法及心脏穿刺采血法进行采血实验。结果眼眶后静脉采血法:采血0.2—0.3 mL/次,每只大鼠3—4次/d。颈静脉采血法:采血0.2—0.4 mL/次,每只大鼠6—8次/d。心脏穿刺采血法:采血0.3—0.4 mL,每只大鼠3—5次/d。结论三种采血方法取血量相对比较大,血液标本质量高,更利于大鼠的动物实验,但各有应注意的事项。Objective To study the commonly used three kinds of method for rat venous blood sampling a day or in a short period of time.Method Total 90 male Wistar rats were used.Three methods were used for the blood sampling,angular vein blood sampling method,the jugular vein blood sampling method and the cardiac puncture blood sampling method.Result 0.2-0.3 mL blood can be collected from the angular vein everytime,and 3-4 times a day ; 0.24-0.4 mL blood can be collected from the jugular vein everytime,and 6-8 times a day ; 0.2-0.4 mL blood can be collected from the cardiac everytime,and 3-5 times a day.Conclusion Large volume and high quanlity of blood samples can be collected by the three blood sampling methods,which can be used in the rat experiment.

关 键 词：大鼠 采血方法 多次 

分 类 号：Q95-33[生物学—动物学]