1,25-二羟维生素D3对新生大鼠高氧肺损伤的保护作用及其机制  

Protective mechanism of 1,25-Dihydroxyvitamin D3 on hyperoxia-induced lung injury in neonatal rats

作　　者：李琼[1] 王琳[1] 陈燕[1] 田元[2] 舒荔[1] 孙雪[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院儿科,武汉430022 [2]华中科技大学同济医学院附属协和医院普外科实验室,武汉430022

出　　处：《中华围产医学杂志》2013年第10期585-588,共4页Chinese Journal of Perinatal Medicine

摘　　要：目的 探讨1,25-二羟维生素D3[1,25-Dihydroxyvitamin D3；1,25（OH）2D3]在新生大鼠高氧肺损伤中的作用及可能机制.方法 新生Sprague-Dawley大鼠生后12h内随机分为空气组、高氧组和干预组,每组8只.高氧组和干预组新生大鼠置于高氧箱（氧浓度≥85％）；空气组新生大鼠置于同一室内常压空气中.干预组新生大鼠生后第1天腹腔注射1,25 （OH）2D30.5 μg/（kg,d）,每日1次,连续7 d；空气组和高氧组相应时间给予等体积生理盐水.第7天时,HE染色观察各组大鼠肺组织形态学改变,并行辐射状肺泡计数；实时荧光定量-聚合酶链反应技术检测肺组织肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）、白细胞介素（interleukin,IL）-1β和IL-6mRNA的表达.组间差异比较采用方差分析,两两比较采用LSD检验.结果 （1）体重：高氧组新生大鼠第7天时体重明显低于空气组[（8.48±1.34）g与（12.51±0.47）g,t=8.05,P＜0.05）,而干预组新生大鼠体重[（10.29±1.00）g]明显高于高氧组（t=3.61,P＜0.05）.（2）肺组织病理变化及辐射状肺泡计数：空气组新生大鼠肺组织结构正常；高氧组新生大鼠肺间质炎症细胞渗出、肺泡大小不均,辐射状肺泡计数较空气组减少[（5.6±0.1）个与（6.8±0.2）个,t=21.45,P＜0.05）],干预组新生大鼠辐射状肺泡计数[（6.2±0.1）个]较高氧组增加（t=11.76,P＜0.05）,但仍低于空气组（t=9.69,P＜0.05）.（3）肺组织TNF α、IL-1β和IL-6 mRNA的表达变化：高氧组新生大鼠肺组织TNF-α、IL-1β和IL-6 mRNA的表达水平分别为0.0348±0.0006、0.0269±0.0003和0.0368±0.0006,均明显高于空气组（分别为0.0111±0.0007、0.0040±0.0003和0.0162±0.0007,t分别为56.54、111.12和49.26,P均＜0.05）；干预组新生大鼠肺组织TNF-α、IL-1β和IL-6 mRNA的表达分别为0.0203±0.0009、0.0141±0.0004和0.0251±0.0009,均较高氧组显著降低（t分别为34.44、61.93和27.99,P均＜0.05）,但�Objective To investigate the effects and mechanisms of 1,25-Dihydroxyvitamin D3 [1,25（OH）2 D3] on hyperoxia-induced lung injury of neonatal rats.Methods Neonatal Sprague-Dawley rats were randomly divided into air group,hyperoxia group and 1,25（OH）2D3 group within 12 hours after birth,eight in each group.Rats in air group were exposed to air,while those in hyperoxia and 1,25 （OH） 2 D3 group were exposed to hyperoxia （≥85 ％ oxygen concentration）.Rats in 1,25（OH）2D3 group were injected with 1,25（OH）2D3 0.5 μg/（kg · d） intraperitoneally once a dayfor seven days,meanwhile the rats in the other two groups received 0.9 ％ saline in the same way.All rats were sacrificed on day 7.Lung tissue sections were HE stained in order to assess lung histological changes and lung radical alveolar count （RAC）.The levels of tumor necrosis factor-α（TNF-α）,interleukin（IL）-1β and IL-6 mRNA were measured by real time-polymerase chain reaction.Analysis of variance and LSD test were applied for statistics.Results Compared with the air group,the weight of rats in the hyperoxia group was significantly lower on day 7 [（8.48±1.34） g vs （12.51±0.47） g,t=8.05,P＜0.05],while the weight of rats in 1,25（OH）2D3 group [（10.29±1.00） g] was higher than that in the hyperoxia group （t=3.61,P＜0.05）.Lung tissue structure was normal in the air group.In the hyperoxia group,inflammatory exudation was observed in pulmonary interstitial,the alveolar size was uneven,and the RAC was lower than that in the air group （5.6±0.1 vs 6.8±0.2,t=21.45,P＜0.05）.The RAC in 1,25（OH）2D3 group （6.2±0.1） was significantly increased compared with that in the hyperoxia group （t=11.76,P＜0.05）,but still lower than that in the air group （t=9.69,P＜0.05）.The expressions of TNF-α,IL-1β and IL-6 mRNA in hyperoxia group （0.0348±0.0006,0.0269±0.0003 and 0.0368 ± 0.0006） were higher than those in the air group （0.0111±0.0007,0.0040±0.0003 and 0.0162 ±0.0007,t=56.54,111.12 an

关 键 词：骨化三醇 高氧症 急性肺损伤 细胞因子类 动物 新生 大鼠 

分 类 号：R722.1[医药卫生—儿科]