美托洛尔对急进4300m高原大鼠心肌及血气的影响  

Effect of Metoprolol on Cardiomyocyte Damage and Blood Gas at 4300 m Altitude in Rats

作　　者：王延玲[1,2] 王荣[1,2] 谢华[1] 李文斌[1] 武晓玉[1] 张娟红[1,2] 马骏[1,2] 贾正平[1,2] 

机构地区：[1]兰州军区兰州总医院全军高原损伤防治重点实验室,甘肃兰州730050 [2]兰州大学药学院,甘肃兰州730000

出　　处：《解放军药学学报》2013年第4期283-286,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家科技部重大资助项目;No.2008ZXJ09014-010;全军医药科研"十二五"重点项目;No.BWS12J012;全军医药科研"十二五"面上项目;No.CWS11C231

摘　　要：目的探讨在4300 m海拔条件下美托洛尔对大鼠心肌的影响。方法 Wistar雄性大鼠30只,分为A组(空白对照组)、B组(高原对照组)及C组(高原美托洛尔组)3组。在各处标准饲养3日后眼眶采静脉血测定肌酸激酶(CK);麻醉取动脉血测定血气;取心脏,用比色法测定丙二醛(MDA),超氧化物岐化酶(SOD);用细胞化学苏木素-伊红(HE)染色法观察左心室病理结构的改变。结果血气显示:B组较A组PCO2、PO2、BE均降低(P<0.05),除PCO2的C组较B组并无显著差异,PO2、BE均明显增加(P<0.05)。MDA、CK值显示:B组较A组明显增加(P<0.05),C组较B组明显减少(P<0.05),SOD值显示:B组较A组明显增加(P<0.05),C组较B组明显降低(P<0.05)。病理切片结果显示A组大鼠心肌细胞排列整齐,心肌纤维完整,B组大鼠心肌细胞排列紊乱,肌纤维溶解断裂,C组大鼠心肌细胞排列较整齐。结论在4300m海拔缺氧条件下,大鼠心肌形成氧化损伤,给予美托洛尔后能显著改善损伤,对心肌有保护作用。Objective To investigate the effect of metoprolol at 4300 m altitude on hypoxic cardiomyocyte in rats.Methods 30 Wistar male rates were randomly divided into 3 groups： group A（control group）,group B（high altitude group） and group C（sildenafil group）.Venous blood,arterial blood and heart tissues were taken from each rat after three days of feeding.Blood gas,the activity of superoxide dismutase（SOD） and concentration of malondialdehyde（MDA） in the heart were measured.Histopathological investigation was performed using specimens of heart tissues with routine hematoxylin-eosin（HE） staining.Results Compared with group A,the content of PO2,PCO2 and BE was decreased（P0.05） while the content of PO2 and BE was increased（P0.05）.Compared with group A,the content of MDA,CK in group B was significantly increased（P0.05）,but the activity of SOD in group B significantly decreased（P0.05）.The content of MDA,CK in group C was significantly decreased compared with group B（P0.05）,but the activity of SOD in group C significantly increased.Histopathological results showed that myocardial fiber in group A was in good order,but there was partial necrosis and disorder in group B.Conclusion Metoprolol can protect cardiomyocoyte from damage.

关 键 词：低氧低气压 氧化损伤 肌酸激酶 血气 丙二醛 超氧化物岐化酶 

分 类 号：R961[医药卫生—药理学]