一种人工合成Tuftsin类似肽大鼠组织分布和排泄研究  

Biodistribution and Excretion of a Synthetic Tuftsin-Analog in Rats

作　　者：贺艳琳[1,2] 顾若兰[2] 韩苏[3] 孟志云[2] 邢春宇[2] 甘慧[2] 吴卓娜[2] 朱晓霞[2] 窦桂芳[2] 

机构地区：[1]中南大学药学院,湖南长沙410013 [2]军事医学科学院野战输血研究所,北京100850 [3]南开大学药学院,天津300071

出　　处：《解放军药学学报》2013年第4期328-331,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家科技重大专项课题-"临床前药代动力学技术平台"资助;No.2012ZX09301003-001-007;国家自然基金资助;No.21104095

摘　　要：目的研究Tuftsin类似肽(T肽)单次皮下注射后在大鼠体内的组织分布特点和排泄情况。方法 Wistar大鼠,单次皮下注射剂量21.6 mg.kg-1T肽。采用竞争ELISA方法检测各时间点的组织器官、尿、粪和胆汁中的药物含量。结果组织分布试验结果表明,药物主要分布在大鼠的血清、肾、脾和小肠组织中,除小肠达峰时间为给药后2 h之外,其它药物含量均于给药后15 min达最高;排泄实验结果表明,0～144 h内尿和粪中排泄的原型药物分别占给药总量的2.21%和0.03%;0～96 h内大鼠胆汁中排泄的原型药物为给药总量的0.02%。结论 T肽给药后在Wistar大鼠体内广泛分布,在血清、肾和脾组织中含量较高,在粪和胆汁中检测出T肽的含量很少。Objective To investigate the biodistribution and excretion of a Synthetic Tuftsin-Analog（T peptide） single subcutaneously administrated in rats.Methods Thirty rats were subcutaneously administered T peptide at a dose of 21.6 mg · kg-1.The T peptide levels in normal organs,urine,excrement and bile were determined by competitive ELISA method,respectively.Results T peptide was widely distributed in the tissues of rats.The concentration of T peptide in the serum,kidney,spleen and small intestine was high in rats,and reached the maximum at 15 min except for the small intestine at 2 h.The excretion of T peptide in the urine,excrement and bile amounted to 2.21%,0.03% and 0.02% of the dose,respectively.Conclusion T peptide is widely distributed in the tissues,especially in the serum,kidney and spleen.A small amount of T peptide is detected in the excrement and bile

关 键 词：TUFTSIN T肽 组织分布 排泄 

分 类 号：R285[医药卫生—中药学]