外膜蛋白与鲍曼不动杆菌多重耐药的相关性研究  

Correlative research of outer membrane protein and multidrug-resistant acinetobacter baumannii

作　　者：李一柯 邓俊[2,3] 

机构地区：[1]长沙市第三医院检验科,湖南长沙410015 [2]湖南省第二人民医院检验科 [3]湖南中医药大学临床实验室,湖南长沙410007

出　　处：《现代医药卫生》2013年第17期2595-2596,共2页Journal of Modern Medicine & Health

摘　　要：目的分析外膜蛋白(OMP)与鲍曼不动杆菌多重耐药的相关性,更好地了解OMP在鲍曼不动杆菌多重耐药中的重要性。方法选取从重症监护病房(ICU)患者痰标本中分离的鲍曼不动杆菌50株,用Whonet5.4软件统计50株鲍曼不动杆菌对环丙沙星(CIP)和亚胺培南(IMP)的耐药情况,用变性聚丙烯酰胺凝胶电泳分析8株多重耐药鲍曼不动杆菌OMP表达情况,研究OMP的表达在鲍曼不动杆菌多重耐药中的重要性。结果 ICU患者分离出的鲍曼不动杆菌对CIP的耐药率达到88%,对IMP的耐药率达16%,耐药形势非常严峻;多重耐药的鲍曼不动杆菌均存在一种或多种OMP表达的下降或缺失。结论 OMP在鲍曼不动杆菌多重耐药中起着重要作用。Objective To investigate the correlation between the outer membrane protein（OMP） and muhidrug-resistant acinetobacter baumannii （ MDR-Ab ）, and to understand the importance of OMP in acinetobacter baumannii muhidrug resistance better. Methods 50 stains of acinetobacter baumannii separated from sputum samples of patients in intensive care unit （ICU） were selected, drug resistance situation of 50 strains of acinetobacter baumannii to ciprofloxacin （CIP） and imipenem （IMP） was analyzed statistically by Whonet5.4 software. The expression situation of MDR-Ab OMP in 8 strains of acinetobacter baumannii was analyzed by modified polyacrylamide gel electrophoresis to study the importance of OMP expression in multidrug resistance of acinetobacter baumannii. Results Drug resistance rate of acinetobacter baumannii strains separated from patients in ICU to CIP reached 88%, and the drug resistance rate to IMP reached 16% with serious drug resistance situation. MDR-Ab had one or several kinds of decline or loss of OMP expression. Conclusion OMP plays an important role in multidrug resistance of acinetobaeter baumannii.

关 键 词：细菌外膜蛋白质类 鲍氏不动杆菌 药物耐受性 环丙沙星 亚胺培南 

分 类 号：R37[医药卫生—病原生物学]