K型温敏雄性不育小麦的遗传模式分析  

Genetic analysis on thermo-sensitive cytoplasmic male sterile wheat lines with Aegilops kotschyi

作　　者：钱焕焕[1] 张玲丽[1] 杜万里[1] 庞玉辉[1] 王亮明[1] 胡甘[1] 宋喜悦[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2013年第9期49-54,60,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(31271792);陕西省自然科学基金项目(2011JM3007)

摘　　要：【目的】明确K型温敏雄性不育小麦的配子传递方式及遗传模式。【方法】以K型1B/1R小麦雄性不育系K3314A,K型非1B/1R雄性不育系A731和A116,保持系116B、731B及恢复系KR783、桑榆3号和WM5-5为材料,构建了4个群体K3314A//KR783/731B、A731//KR783/731B、A116//A116/桑榆3号和A116//116B/WM5-5,分别调查其亲本及后代的自交结实率和单倍体发生的频率,结合植物数量性状主基因+多基因混合遗传模型进行遗传分析。【结果】1BS染色体片段代换了1RS染色体片段后,非1B/1R类型不育系及其后代不产生单倍体;不育系核内基因主要由雌配子传递,属配子体不育类型;育性受2对加性-显性-上位性主基因+加性-显性-上位性多基因共同控制,且2对主基因控制育性的作用相当,在B2群体中主基因的遗传率最高。【结论】杂合1B/1R核型要比纯合1B/1R核型产生的单倍体频率高;在K型不育系育性相关基因定位的群体选择中,只有组配不育系//保持系/恢复系的后代,才会出现不育和可育的分离;该类型小麦温敏雄性不育系中有2个主效基因的存在,同时受多基因的修饰作用。[Objective] This study aimed to clarify gamete transfer mode and inheriting model of K-type TCMS wheat. [Method] Four groups, K3314A//KR783/731B, A731//KR783/731B, A116//A116/Sangyu 3 ~ and A116//116B/WM5-5 were built. The frequencies of haploid and the self-setting rates of parents and progenies were investigated. Genetic analysis was carried out with mixed inheritance model of quantita- tive trait gene＋polygenes. [Result] The sterile line and its progenies could not produce haploids when 1RS chromosome segment was substituted by 1BS chromosome segment. It was a gametophytic type due to its nuclear gene mainly passing through female gamete and its fertility was determined by two pairs of genes, namely additive-dominance-epistasis major genes＋ additive-dominance-epistasis polygenes, and they had the equal effects on fertility controlling. B2 group had the highest major gene heritability. [Conclusion] The heterozygous 1B/1R karyotype had a higher frequency of haploid than pure 1B/1R karyotype. Group selec- tion for fertility-related genes of K-type TCMS should only consider the combination of sterile line//main-tenance line/restorer line. There were two major genes in this TCMS wheat line and they were modified by polygenes.

关 键 词：K型温敏雄性不育 配子体不育 遗传模型 

分 类 号：S512.103.3[农业科学—作物学]