甘肃河西走廊苹果蠹蛾种群遗传分化研究--基于COI基因序列分析  

COI gene analysis based genetic differentiation of Cydia pomonella(Lepidoptera:Tortricidae)populations in Hexi Corridor

作　　者：李玉婷[1] 王康[1] 郑燕[1] 门秋雷[1] 钱路[2] 安榆林[2] 冯纪年[1] 张雅林[1] 陈茂华[1] 

机构地区：[1]西北农林科技大学植物保护学院,农业部西北黄土高原作物有害生物综合治理重点实验室,植保资源与病虫害治理教育部重点实验室,陕西杨凌712100 [2]江苏出入境检验检疫局动植食中心,江苏南京210001

出　　处：《西北农林科技大学学报（自然科学版）》2013年第9期85-90,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(31071687,31272036);教育部高等学校博士学科点专项科研基金项目(博导类)(20110204110001);教育部留学回国人员科研启动基金项目;公益性行业(农业)科研专项(200903042-03);国家科技支撑计划课题(2012BAK11B03);陕西省“13115”科技创新工程重大科技专项(2009ZDKG-06);西北农林科技大学人才引进项目

摘　　要：【目的】揭示河西走廊苹果蠹蛾不同地理种群间的联系,分析地理种群间的序列变异、遗传多样性和遗传分化。【方法】采用PCR和基因测序技术,扩增并分析了河西走廊8个地理种群132个苹果蠹蛾个体的线粒体细胞色素氧化酶Ⅰ亚基(COI)基因片段,应用DnaSP 5.10计算单倍型多样性指数(Hd)、核甘酸多样性指数(Pi)和Tajima’s D值,采用ZT软件包进行Mantel检验,分析种群间遗传距离与地理距离的相关性,利用软件Arlequin 3.11计算成对种群间的固定系数(Fst)。【结果】在获得的132条序列中共发现了8个变异位点和5个单倍型,其中3个单倍型为种群共享单倍型。总体单倍型多样性指数为0.578,种群内单倍型多样性为0.000~0.700。各种群的Tajima’s D值中性检验符合中性突变,说明河西走廊苹果蠹蛾在历史上没有出现群体扩张,群体大小稳定。Fst值表明,河西走廊苹果蠹蛾种群间具有一定程度的遗传分化,而各地理种群的遗传距离与地理距离无显著的相关性。【结论】河西走廊苹果蠹蛾遗传多样性较低,且地理种群间有一定程度的遗传分化。[Objective] The aim of this study was to clarify the genetic relationship of Cydia pomonella populations collected from different geographic locations in Hexi Corridor,China, and to analyze their ge- netic diversity and genetic differentiation based on cytochrome oxidase subunit I （COI） gene fragments. [Method] PCR and gene sequencing technology were used to obtain the COI gene sequences from 132 indi-viduals sampled in 8 geographic locations in Hexi Corridor. DnaSP 5.10 was used to calculate the molecular genetics parameter. Mantel test was conducted by ZT software package was used to analyze the correlation between genetic distance and geographical distance. Arlequin 3.11 was used to calculate the pairwise Fst value. [Result] Among the obtained 132 COI sequences,8 variable sites and 5 haplotypes were observed, and 3 haplotypes were shared among the populations. The total haplotype diversity （Hd） was 0. 578,while it varied from 0. 000 to 0. 700 within populations. Tajima^s D value neutrality test suggested that there was no population expansion for the codling moth in history. Fst value indicated that there was genetic differen- tiation among codling moth populations in Hexi Corridor. The genetic distance was not significantly corre- lated with the geographic distance between populations. [Conclusion] The genetic diversity of codling moth in Hexi Corridor was low, and there was a certain degree of genetic differentiation between populations.

关 键 词：苹果蠹蛾 COI基因 地理种群 遗传分化 

分 类 号：S436.611.2[农业科学—农业昆虫与害虫防治]