平菇汁对野蘑菇生长的研究  

Influence of Pleurotus Ostreatus Juice on the Growth of Agaricus Arvensis

作　　者：赵磊[1] 陈有君[1] 李国光[1] 李春玲[1] 李立民[1] 

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

出　　处：《农产品加工（下）》2013年第9期72-75,共4页Farm Products Processing

摘　　要：野蘑菇具有很高的营养和药用价值,但人工培养时生长缓慢、老化快、产量低,使得在菌剂等实际生产应用方面受到限制。结合有关文献资料,发现平菇汁中有不知名组分对野蘑菇菌丝生长有显著的促进作用,旨在找出并确定这种物质及其最适生长浓度。分别以水、乙醇、丙酮为透析液,MWCO为8 000～14 000的透析袋透析等体积蘑菇汁,DNS法测还原糖、双缩脲法测蛋白质、酸性染料比色法测生物碱、苯酚-硫酸法测总糖、甲醛法测氨为测定手段,菌丝直径为测量指标。蛋白质多的组分加入培养基中野蘑菇菌丝生长迅速,生物碱多的组分加入培养基野蘑菇菌丝生长缓慢。平菇汁中有类蛋白物质起促进野蘑菇菌丝生长作用,生物碱类物质起抑制野蘑菇菌丝生长作用。Agaricus arvensis has well nutritional and medicinal value,but because of its slow growth,rapid aging,low yield,its practical application is still limied.Now with the literature material,it finds that there is some unknown components in Pleurotus ostreatus juice on Agaricus arvensis mycelium growth has a significant role,designed to identify and determine the substance,and its optimum growth concentration.This experiment is to analyze the same volume Pleurotus ostreatus juice respectively in water,ethanol,acetone,in dialysis bag whose MWCO is 8 000～14 000,by DNS method of reducing sugars,biuret method for measuring protein,acidic dye colorimetry measured alkaloids,phenol-sulfuric acid method of measuring total sugar measuring ammonia,formaldehyde method for the determination of means,and the hypha growth diameter,combining with the development situation,get the observed phenomenon：components have more protein joined medium in where Agaricus arvensis hypha growth is rapid,components have more alkaloid joined medium in where Agaricus arvensis hypha growth is slow,alkaloid joined medium part of the Agaricus arvensis hypha growth is slow,so come to the conclusion： there are classes of protein substances play a promoting effect on Agaricus arvensis mycelium growth,alkaloids substances inhibiting Agaricus arvensis hypha growth role.

关 键 词：野蘑菇 平菇汁 分离 

分 类 号：S646[农业科学—蔬菜学]