黑逍遥散对东莨菪碱致痴呆小鼠模型的影响  

Effect of Heixiaoyao Powder on Mice Model of Senile Dementia Induced by Scopolamine

作　　者：吴红彦[1,2] 李海龙[1,2] 张云[3] 赵鹏[1] 顾静[1] 车敏[1] 王虎平[1,2] 

机构地区：[1]甘肃中医学院,甘肃兰州730000 [2]甘肃省中药药理与毒理学重点实验室,甘肃省中药新产品创制工程实验室,甘肃兰州730000 [3]甘肃省中医院,甘肃兰州730050

出　　处：《中国中医药信息杂志》2013年第10期35-37,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81060284);甘肃省财政厅高校基本科研业务费项目(甘财教〔2009〕192号)

摘　　要：目的观察黑逍遥散对东莨菪碱致痴呆小鼠模型的影响,探讨其防治痴呆的作用机制。方法昆明种小鼠50只,随机分为空白组、模型组、黑逍遥散组、逍遥散组、精简方组,每组10只。采用氢溴酸东莨菪碱腹腔注射诱发小鼠记忆障碍建立模型,各药物组每日给予相应的原药材水煎液2.314 g/kg,空白组和模型组给予等量生理盐水,每日灌胃1次,连续灌胃10 d。给药后第6、8日采用水迷宫法、跳台法及避暗法进行行为学能力训练,给药后第10日进行行为学能力测试,完毕后处死动物,采集脑组织并测定脑组织中乙酰胆碱酯酶(AchE)和胆碱乙酰转移酶(ChAT)的活性。结果与模型组比较,黑逍遥散组、逍遥散组及精简方组均能减少水迷宫测试中错误次数,缩短潜伏时间;减少在跳台、避暗测试中的电击次数,延长潜伏期时间;均能升高脑组织匀浆ChAT活性,降低AchE活性(P<0.01,P<0.05),其中以黑逍遥组效果最为明显。结论黑逍遥散及其类方具有防治老年性痴呆的作用。Objective To explore the mechanisms and the significance of compatibility of Heixiaoyao Powder by observing the effect of Heixiaoyao Powder on mice model of senile dementia induced by scopolamine. Methods Kunming mice were randomly divided into five groups including control group, model group, Heixiaoyao Powder group, Xiaoyao powder group and streamlined formula group, with 10 mice of each group. Scopolamine hydrobromide intraperitoneal injection was adopted to make memory impairment in mice model, and followed by drugs treatment with corresponding decoction of the crude drug 2.314 g /（kg·d）. Control group and model group were given normal saline orally once a day, all groups were treated for 10 days continuously. After 6 and 8 days of the administration, water maze test, stair jumping test and darkness avoidance test were conducted to test behavior proficiency, and the activity of acetylcholinesterase （AchE） activity and choline acetyltransferase （ChAT） in brain tissue were tested also. Results Compared with model group, Heixiaoyao Powder group, Xiaoyao Powder group and streamlined formula group could significantly reduce the error number and shorten latency in the water maze test, decrease the time of electrical shocks and prolong the incubation period of time in both stair jumping test and darkness avoidance test, and could increase ChAT activity and lower AchE activity in brain tissue of mice （P〈0.01 or P〈0.05）, especially the effects of Heixiaoyao Powder group. Conclusions Heixiaoyao Powder and its analogous formula have the function of preventing and treating senile dementia.

关 键 词：黑逍遥散 老年性痴呆 动物行为 氢溴酸东莨菪碱 乙酰胆碱酯酶 胆碱乙酰转移酶 小鼠 

分 类 号：R285.5[医药卫生—中药学]