参龙汤对脑缺血大鼠学习记忆能力及海马Bcl-2和Bax表达的影响  

Effect of Shenlongtang on Learning and Memory Ability and Expression of Bcl-2 and Bax in Hippocampus of Cerebral Ischaemia Rats

作　　者：陈利平[1] 王发渭[1] 孙志高[1] 许成勇[1] 

机构地区：[1]中国人民解放军总医院海南分院,海南三亚572004

出　　处：《中国中医药信息杂志》2013年第10期41-43,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市科技计划研发攻关基金(Z111107067311022)

摘　　要：目的观察参龙汤对脑缺血大鼠学习记忆能力及大脑海马区Bcl-2和Bax表达的影响。方法利用双侧颈总动脉结扎的方法制备慢性脑缺血大鼠模型,50只大鼠随机分为对照组、模型组、脑复康组及参龙汤大、小剂量组。造模后药物处理4周,采用Morris水迷宫实验观察大鼠逃避潜伏期的时间及跨越平台的次数,采用免疫组化方法观察大鼠海马Bcl-2和Bax表达的变化。结果模型组大鼠逃避潜伏期时间较对照组明显延长,跨越平台次数较对照组明显减少(P<0.01)。参龙汤大剂量组大鼠的逃避潜伏期时间较模型组明显缩短(P<0.05),跨越平台次数明显增加(P<0.05);参龙汤大剂量组大鼠海马Bax蛋白阳性表达显著低于模型组(P<0.05),Bcl-2蛋白阳性表达则显著高于模型组(P<0.01)。结论参龙汤可以改善脑缺血大鼠学习记忆能力,其作用机理可能与增加脑缺血大鼠海马Bcl-2蛋白阳性表达、减少Bax蛋白阳性表达有关。Objective To investigate the effect of Shenlongtang decoction on learning and memory ability and expression of Bcl-2 and Bax on rats hippocampus with cerebral ischemia model. Methods The cerebral ischemia animal model was established by two-vessel occlusion. Fifty rats were randomly divided into control group, model group, pisacetam group, Shenlongtang groups （low and high dosage）. After modeling, rats were administrated with corresponding drugs for 28 days, the learning and memory ability was tested with Morris water maze, the number of positive cells and the integral optical density of the immunostaining on Bcl-2 and Bax protein expression in the hippocampus was detected. Results With Morris water maze test, the latency time increased significantly and times of searching submerged platform decreased significantly in the model group, the difference was significant compared with control group （P〈0.01）. Compared with model group, the latency time of Shenlongtang high dosage group decreased significantly and times of searching submerged platform increased significantly （P〈0.05）. Compared with model group, the expression of positive cells number of Bax protein expression in the hippocampus area was significantly increased （P〈0.05）, the Bcl-2 protein expression in the hippocampus region was significantly decreased （P〈0.01）. Conclusion Shenlongtang could significantly improve the learning and memory ability of cerebral ischemia rats, the mechanism may be related to regulating the expression of Bcl-2 and Bax.

关 键 词：参龙汤 脑缺血 学习记忆能力 BCL-2蛋白 BAX蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]