桃仁膝康丸HPLC指纹图谱研究  

HPLC Fingerprint of Taoren Xikang Pills

作　　者：李洁 罗石任[2] 王秀真[2] 

机构地区：[1]河南省正骨研究院,河南洛阳471002 [2]河南中医学院,河南郑州450008

出　　处：《中国中医药信息杂志》2013年第10期59-62,共4页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立桃仁膝康丸高效液相色谱指纹图谱,提供较全面评价、控制制剂质量的方法。方法采用高效液相色谱-紫外检测法分析10批桃仁膝康丸的甲醇提取物。色谱柱Agilent C18(4.6 mm×250 mm,5μm);流动相:乙腈-水梯度洗脱;流速:1.0 mL/min;检测波长:203 nm。结果建立了桃仁膝康丸高效液相色谱指纹图谱,共标示26个共有指纹峰,10批被测样品的色谱指纹图谱整体相似度在0.9以上。结论本方法准确可靠,可有效控制桃仁膝康丸的制剂质量。Objective To establish the HPLC fingerprint of Taoren Xikang pills and provide the method for the quality control and evaluation of the preparation. Methods Methanol extracts of ten batches of Taoren Xikang pills were analyzed with HPLC-UV method. Analysis was performed on C18 column （4.6 mm× 250 mm, 5 μm） with the mobile phase of acetonitrile-water gradient eluted at the flow rate of 1.0 mL/min. The detection wavelength was 203 nm. Results A standard HPLC fingerprint procedure was developed for Taoren Xikang pills with 26 common peaks. The similarity of 10 batches of Taoren Xikang pills was not lower than 0.9. Conclusion The method is accurate, repeatable and can be used for the quality control of Taoren Xikang pills.

关 键 词：桃仁膝康丸 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]