HPLC法测定绞股蓝中人参皂苷Rb_1的含量  

Determination of Ginsenoside Rb_1 in Gypenoside Tablets

作　　者：陈英 

机构地区：[1]福建中鹭医药有限公司,福州350001

出　　处：《海峡药学》2013年第9期84-86,共3页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC法测定绞股蓝总甙片中人参皂苷Rbl的含量。方法色谱柱为AgilentExtend—c18（5μm，4．6mmx250mm），流动相为乙腈-水线性梯度洗脱，流速为I．OmL·Min-1。紫外检测波长为203nm，柱温35℃。结果人参皂苷Riol在0．422—2．5321μg．mL-1。范围内线性良好，平均回收率为97．56％，RSD为1．05％（n=6）。结论该方法结果准确、重复性好，可用于绞股蓝总甙片的质量控制。OBJECTIVE To estabilish an HPLC method for quantitative determining of ginsenoside Rbl in Gypenoside Tablets. METHODS Separation was accomplished on the Agilent Extend-Cls column（51m,4. 6mm x 250mm） ,with aeetonitrile-water as mobile phase by gradient elution. The flow rate was 1.0mL min -1 and the de-tection wavelength was set at 203nm. The column temperature was at 35~C. RESULTS The method offered good linearity 0. 422 ~2. 5321xg. mL-1 for ginsenoside RbI （ r =0. 9997）. The average recovery rate was 97.56% （ RSD = 1.05% ,n = 6）. CONCLUSION The method was accurate and repeatable, and can be used for quality control of Gypenoside tablets.

关 键 词：绞股蓝总甙片 人参皂苷RB1 高效液相法 

分 类 号：R927.2[医药卫生—药学]