微腔型PCR芯片用于法医DNA分析的初步研究  

Application of micro-cavity PCR chip in forensic DNA analysis

作　　者：董军磊[1] 欧元[2,3] 李彩霞[2] 秦奎伟[3] 刘琳[1] 姜伯伟[4] 赵兴春[2] 叶健[1,2] 

机构地区：[1]中国人民公安大学,北京100038 [2]公安部物证鉴定中心,北京100038 [3]北京理工大学,北京100081 [4]中科院上海应用物理研究所,上海201800

出　　处：《刑事技术》2013年第5期22-24,共3页Forensic Science and Technology

摘　　要：目的为了克服传统PCR热循环仪体积大,运行电压高,耗时长,只能在实验室中应用的缺点,研究了一种微腔型PCR芯片,以期实现现场对STR片段的复合扩增。方法采用在PCR反应缓冲液中加入不同浓度的BSA溶液对芯片进行表面优化处理的方法及不同酶量优化实现对STR片段的有效扩增。结果使用浓度为0.5mg/mL的BSA可得到清晰完整的STR分型结果;加大酶量有益于扩增效率的提高。结论该种微腔型PCR芯片经初步优化后可有效地对STR片段进行复合扩增,经进一步优化可真正实现法医DNA分析的更加微量化和快速化。Objective To develop a rapid method for DNA analysis by using micro-cavity PCR chip. Methods By adding different concentrations of BSA solution and TaqGold polymerase in the PCR reaction buffer,the surface of the chip was optimized to achieve efficient amplification of the STR fragments. Results Complete STR genotyping results were obtained with a concentration of 0.5mg/ml of BSA. Conclusion The optimized micro-cavity PCR chip was effective for multiplex amplification of STR fragments, and it is possible to achieve a rapid forensic DNA analysis at the crime scene through further optimization.

关 键 词：法医遗传学 PCR芯片 DNA分析 

分 类 号：DF795.2[医药卫生—法医学]