硫化氢上调ABCA1表达促进泡沫细胞胆固醇流出的实验研究  

The study of hydrogen sulfide up-regulates the expression of ABCA1 and promotes the cholesterol efflux in foam cells

作　　者：李国术 何平平[2] 王波[3] 周寿红[2] 欧阳新平[2] 

机构地区：[1]衡阳市中心医院急诊科,湖南衡阳421001 [2]南华大学心血管疾病研究所/生理学教研室,湖南衡阳421001 [3]南华大学附属第一医院麻醉科,湖南衡阳421001

出　　处：《重庆医学》2013年第30期3596-3598,共3页Chongqing medicine

基　　金：国家自然科学基金项目(81300158);湖南省科技厅项目(2013FJ3027);湖南省教育厅科研项目(13B103)

摘　　要：目的探讨外源性硫化氢(H2S)对泡沫细胞胆固醇流出和三磷酸腺苷结合盒转运体A1(ABCA1)表达的影响。方法氧化低密度脂蛋白孵育RAW264.7细胞诱导泡沫化,H2S供体硫氢化钠处理细胞,测定胆固醇流出率,高效液相色谱测定细胞内游离胆固醇(FC)、胆固醇酯(CE)和总胆固醇(TC)浓度。实时定量PCR和蛋白印迹法检测泡沫细胞中ABCA1mRNA和蛋白表达。结果与泡沫细胞组相比,外源性H2S以时间和浓度依赖的方式增加泡沫细胞胆固醇流出(P<0.05),降低细胞中TC、FC和CE及CE/TC水平(P<0.05),上调细胞中ABCA1表达。结论外源性H2S上调泡沫细胞中ABCA1表达,促进胆固醇流出。Objective To investigate the effect of hydrogen sulfide （H2S） on the cholesterol efflux and ATP-binding cassette transporter A1 （ABCA1） expression in foam cells .Methods RAW 264 .7 macrophages were incubated with oxidized low density lipoprotein to induce foam cells .Foam cells were incubated with H2S donor sodium hydrosulfide .Cholesterol efflux from macropha-ges was tested by labed cholesterol .The cellular levels of free cholesterol （FC） ,cholesterol ester （CE） and total cholesterol （TC） were measured by high performance liquid chromatography assays .The mRNA and protein expressions of ABCA1 were detected by Real-time PCR and Western blot .Results Compared with the foam cells ,the rates of cholesterol efflux were significantly in-creased ,the levels of TC ,FC ,CE and CE/TC ratio were significantly decreased（P〈0 .05） and expression of ABCA1 was signifi-cantly increased by treatment with H2S in dose-and time-dependent manner（P〈0 .05） .Conclusion H2S up-regulates of expres-sion ABCA1 and promotes cholesterol efflux in RAW 264 .7 macrophage-derived foam cells .

关 键 词：硫化氢 胆固醇流出 三磷酸腺苷结合盒转运体A1 

分 类 号：R965[医药卫生—药理学]