氯苄基砜衍生物含量测定方法学研究  

Method Study on Determination of Content of Chloro-benzyl Sulfone Derivative

作　　者：陈莉[1,2] 杨建云[2] 肖炳坤[2] 甄蓓[2] 黄荣清[2] 

机构地区：[1]中南大学药学院,长沙410013 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《现代仪器与医疗》2013年第5期63-65,74,共4页Modern Instruments & Medical Treatment

基　　金：国家自然科学基金项目(NO.81072613);国家"重大新药创制"综合性新药研究开发技术大平台-药物分析及质量控制研究分题(NO.2012ZX09301003–001–010)

摘　　要：目的建立氯苄基砜衍生物（CBS）含量测定高效液相色谱法,并进行方法学研究,验证方法的可靠性及科学性;方法色谱柱为Phenomenex LUNA C18250×4.6mm 5μm;流动相为乙腈-0.1%三氟乙酸（45：55,V/V）;流速1.0mL.min-1;检测波长279 nm;柱温40℃;进样体积20μL;结果精密度RSD为0.6%,线性范围为10μg·mL-1~100μg·mL-1（r2=0.9990）,CBS平均含量为100.91%（n=6）,有关物质与主峰分离良好。结论该方法简便、灵敏、准确,可用于CBS的质量控制。Objective To establish method for determination of content of Chlorobenzyl sulfone Derivative（CBS） by HPLC,and prove that the method is reliable and durable Methods The Phenomenex LUNA-C 18（250mm 4.6mm,5μm） was used,the mobile phase was acetonitrile ：0.1% trifluroacetic acid in water（45：55,v/v）,the flow rate was 1.0ml/min,the ultraviolet detection was at 279 nm,The column temperature was 40 ℃,the injection volume was 20 μl.Result The accuracy RSD was 0.6%,the linear range was 10 μg·ml-1~100 μg·ml-1（r2=0.9990）,the average content of CBS was 100.91%,RSD was 0.93%（n=9）.Conclusion This method is simple,sensitive and accurate.It can be used for the quality control of CBS.

关 键 词：氯苄基砜衍生物 方法学研究 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]