“新吉富”罗非鱼消化道指数及2种蛋白酶活性研究  

Studies on the Digestive Tube Index and the Activity Distribution of Two Digestive Protease Activity in NEW GIFT Tilapia(Oreochromis niloticus)

作　　者：吴斌[1] 樊海平[1] 钟全福[1] 翁祖桐 梁萍[1] 陈度煌[1] 

机构地区：[1]福建省淡水水产研究所,福建福州350002 [2]福建省水产技术推广总站,福建福州350003

出　　处：《福建农业学报》2013年第8期727-730,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省海洋与渔业厅重点项目(闽海渔[2010]2-12号);福建省科技计划项目(2011N0006);现代农业产业技术体系建设专项(CARS-49;CARS-46-35);福建省种业创新与产业化工程项目(FJZY-罗非鱼)

摘　　要：开展“新吉富”罗非鱼消化道指数以及胃蛋白酶和胰蛋白酶活性动态变化研究，结果表明，“新吉富”罗非鱼比肠长为4.921±0.187，比肠质量为0.018±0.002，比肝质量为0.014±0.003，比内脏质量为0.039±0.007，表现为偏植食性为主的杂食性鱼类特征。空腹再投喂后0～96 h ，胃蛋白酶活性于12h 增加至最大值167.872 U ，投喂12～96 h 其活性降低，特别是12～24 h ，减少幅度较大。同时检测其肝脏、前肠、中肠、后肠中胰蛋白酶活性动态变化情况，各组织的胰蛋白酶活性比较，肝＜中肠＜前肠＜后肠，各部位的胰蛋白酶活性总体呈先增加达最大值后，再减小的趋势，各部位胰蛋白酶活性差异显著（P＜0.05）。Digestive tube index and the activity distribution of pepsin and trypsin In NEW GIFT Tilapia （Oreochromis niloticus） were studied . It was found that Oreochromis niloticus had intestinal length （IL ） /total length （ TL ） of 4.921 ± 0.187 , intestinal weight （ IW ） /body weight （BW ） of 0.018 ± 0.002 , and hepatopancreas weight （HW ） /BW of 0.014 ± 0.003 , splanchnic weight （SW ） /BW of 0.039 ± 0.007 . The result showed that Oreochromis niloticus was a kind of heterophagous fish with partial phytophagy food habit .After starvation and refeeding , Pepsin activity increased to a maximum of 167.872 U at 12h .However ,it reduced from 12h to 96h ;especially 12h to 24h ,the decrease rate was significant .Trypsin activities of hepatopancreas ,foregut , midgut and hindgut were tested .The specific activity of trypsin in four organs was in the order of hepatopancreas 〈midgut〈foregut〈hindgut. The Trypsin activities in four parts increased up to a maximum during the first stage, then decreased smoothly, trypsin activity of each tissues was significant difference （P〈0.05）.

关 键 词：“新吉富”罗非鱼 消化道指数 胃蛋白酶 胰蛋白酶 

分 类 号：S965[农业科学—水产养殖]