嗜水气单胞菌外膜蛋白ISCOM疫苗免疫欧洲鳗鲡后抗体分泌细胞变化  

European Eel Antibody Secreting Cells Changes After Immunize by Aeromonas hydrophila MOMP ISCOM Vaccine

作　　者：林晨韬[1] 黄春兰[1] 李艳虹[1] 李盼[1,2] 宋铁英[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003 [2]福建农林大学动物科学学院,福建福州350002

出　　处：《福建农业学报》2013年第9期859-863,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2011J05054);福建省科技重大专项(2010NZ0002-3);福建省农业科学院引进海外人才科研启动基金项目(HWRC2011-02)

摘　　要：制备嗜水气单胞菌MOMP-ISCOMs疫苗,使用该疫苗口服和注射免疫欧洲鳗鲡,分别于口服或注射免疫后第14、28d分离欧洲鳗鲡的皮肤和肾脏细胞,使用ELISPOT技术分析其中抗体分泌细胞ASC的数量变化。结果显示:2种免疫方式均能不同程度增加皮肤和肾脏中免疫细胞的数量;其中口服免疫组非特异性免疫细胞数量增加速度较快,注射免疫组特异性免疫细胞数量增加更多。Aeromonas hydrophila MOM P-ISCOMs vaccine was prepared , and the vaccine was given to European eel by oral or injection .The European eel skin and kidney cells were separated and isolated after immunization 14 and 28 days respectively ,in which the antibody secreting cells （ASC） ,were analyzed by ELISPOT technique .The results showed that the two immune ways could affect the number of immune cells in skin and kidneys in different amount of non-specific immune cells increased rapidly in oral immunization group , and specific immune cells increased more obviously in injection group .

关 键 词：嗜水气单胞菌 欧洲鳗鲡 外膜蛋白ISCOMs疫苗 皮肤 肾脏 ASC 

分 类 号：S942[农业科学—水产养殖]