小菜蛾泛素基因的克隆与分析  

Cloning and Analysis of Ubiqitin Genes of Plutella Xylostella (L.)

作　　者：张宏[1] 杜亚琼[1] 李凤良[2] 周洲[1] 顾曙余[1] 程罗根[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210023 [2]贵州省农业科学院植物保护研究所,贵州贵阳550006

出　　处：《南京师大学报（自然科学版）》2013年第3期116-119,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31071974);江苏省研究生科研创新计划项目

摘　　要：运用RT-PCR技术,从小菜蛾幼虫总RNA反转录产物中扩增出泛素基因的cDNA序列.利用RNA干扰(RNAi)技术将一段体外合成的与小菜蛾泛素延伸蛋白基因UBA52同源的dsRNA注入四龄幼虫体内,以抑制小菜蛾UBA52基因的表达.半定量RT-PCR结果显示,72 h后沉默组小菜蛾幼虫体内UBA52基因的mRNA表达量显著下调.本实验成功沉默小菜蛾泛素基因,此RNAi干扰体系的建立为利用此技术分析小菜蛾及其他昆虫基因的功能提供了参考.The cDNA sequences of ubiquitin genes were isolated from PluteUa xylostella by reverse transcription polymerase chain reaction（RT-PCR）. Using RNA interference （RNAi）, the in vitro systhesized double-stranded RNA （dsRNA） homologous to the UBA52 gene was microinjected into 4th instar larvae of P. xylostella to surpress the gene ex- pression. The results of semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） demonstrated that UBA52 gene expression was reduced sharply at 72h after injection. The ubiquitin gene was silenced successfully in this experiment, and the establishment of RNAi technique can help reveal the gene function of P. xylostella and other insects.

关 键 词：小菜蛾 泛素基因家族 RNA干扰 基因沉默 

分 类 号：Q969.42[生物学—昆虫学]