大白菜WRKY转录因子的全基因组鉴定与分析  

Genome-wide identification and analysis of WRKY transcription factors in Chinese cabbage

作　　者：蒋明[1] 苗立祥[2] 张豫超[2] 管铭[1] 潘小翠[1] 

机构地区：[1]台州学院生命科学学院,浙江临海317000 [2]浙江省农业科学院园艺研究所,浙江杭州310021

出　　处：《浙江农业学报》2013年第5期980-986,共7页Acta Agriculturae Zhejiangensis

基　　金：浙江省公益性技术应用研究计划项目(2012C32011);台州市科技计划资助项目(121KY16);浙江省自然科学基金项目(LY13C150003)

摘　　要：WRKY转录因子是一类重的调控分子，在高等植物中以基因家族的形式存在。试验以大白菜基因组数据为材料，利用生物信息学手段对WRKY转录因子进行了全基因组鉴定与分析。结果表明，大白菜至少有134个WRKY转录因子，蛋白序列长度为143～1082；基因组DNA全长880—9099bp，具1～15个内含子。染色体定位结果表明，10条染色体上均有WRKY转录因子基因的分布，A3染色体上最多（26），A10染色体分布最少（4）。大白菜WRKY转录因子可分为3大类，I类、Ⅱ类和Ⅲ类的数量分别为27，83和24，它们处于进化树的不同分支。除保守的WRKYGQK和常见变异序列WRKYGKK外，新发现WRKDGQK，WRKNGQK和WMKYGQK等3种WRKY结构域类型。WRKY transcription factors act as important regulatory molecules, and exist in the form of gene family in higher plants. In this study, genome-wide identification and analysis of WRKY transcription factors in Brassica rapa ssp. pekinenzis were performed with bioinformatic methods using genomic data. We identified 134 WRKY transcrip- tion factors with protein sequence length varying from 143 to 1 082. Their full length genomic DNA were 880 to 9 099 bp in size with 1-15 introns. Location analysis of WRKY genes indicated that they distributed on all 10 chromo- somes. Most of the WRKYs were present on chromosome A3 （26）, while the fewest on A10 （4）. WRKY transcrip- tion factors can be divided into three major groups located at different branches of the phylogenetic tree. The gene numbers of I , ]I and ]lI groups were 27, 83 and 24, respectively. Three new types of WRKY domains, WRK- DGQK, WRKNGQK and WMKYGQK, were indentified in addition to conserved WRKYGQK and its common variant WRKYGKK sequences.

关 键 词：大白菜 WRKY转录因子 全基因组 序列分析 

分 类 号：Q78[生物学—分子生物学] S634.1[农业科学—蔬菜学]