白术真菌病害的分离鉴定  

Isolation and identification of fungal pathogens of Atractylodes macrocephala

作　　者：檀国印[1] 杨志玲[1] 袁志林[1] 杨旭[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《浙江农业学报》2013年第5期1050-1055,共6页Acta Agriculturae Zhejiangensis

基　　金：国家林业局948项目(2011464;2011-2013)

摘　　要：2011年6—8月份在浙江磐安地区采集到白术病害根、茎和叶片样本，经过室内分离试验得到4个根茎真菌分离物（分别记为R1～R4）与8个叶片真菌分离物（分别记为L1～L8）。依据科赫法则进行致病性测定，R1，R2为白术茎腐病病原菌，L3为白术叶斑病病原菌。通过形态学观察及分子鉴定方法确定R1为尖孢镰刀菌Fusariumoxysporura，R2为半裸镰刀菌Fusariumincarnatum，L3为长柄链格孢菌Alternarialongipes。Diseased Atractylodes macrocephala were sampled in Panan, Zhejiang Province during June and August, 2011. Several fungus, 4 from stems （ R1 - R4 ） and 8 from leaves （ L1 - L8 ） , were isolated. The results determined by Koch＇s postulates indicated that R1 and R2 could cause stem-rot disease, while L3 could cause leaf spot on Atrac- tylodes macrocephala. Through morphological and molecular identification, R1, R2 and L3 were Fusarium oxyspo- rum, Fusarium incarnatum and Alternaria longipes, respectively.

关 键 词：白术 真菌病害 尖孢镰刀菌 半裸镰刀菌 长柄链格孢菌 

分 类 号：S432.1[农业科学—植物病理学]