电针对MCAO大鼠脑皮质EPO表达和脑血流量的影响  

Effects of electroacpuncture on expression of EPO and cerebral blood flow in rats with MCAO

作　　者：梁超[1] 陈邦国[1] 李昂[1] 

机构地区：[1]湖北中医药大学针灸骨伤学院,武汉430065

出　　处：《中国康复》2013年第5期323-326,共4页Chinese Journal of Rehabilitation

基　　金：教育部高等学校博士学科点专项科研基金(20094230110002)

摘　　要：目的:观察电针疗法对局灶性脑缺血大鼠脑皮层EPO表达和缺血局部组织血流量的影响,探讨其作用机制。方法:SD大鼠80只,随机分为正常组和假手术组各10只、模型组和电针组各30只;模型组和电针组又各按缺血再灌注24h、48h和72h分别分为3个亚组,每组10只。假手术组仅分离血管而不插线栓,模型组和电针组制备大脑中动脉缺血再灌注模型(MCAO),电针组采用电针治疗。采用免疫组化组法及免疫印迹法检测大鼠脑皮质中促红细胞生成素(EPO)蛋白的表达,用激光多谱勒血流仪检测大鼠脑组织血流量(rCBF)的变化。结果:EPO免疫组化及Western结果比较,模型组及电针组EPO阳性细胞及蛋白表达均在脑缺血再灌注24h开始增加,48h达到峰值,72h开始下降,且电针组在3个时间点均高于同时间点模型组(P<0.05,0.01)。rCBF比较,模型组和电针组随着24、48、72h3个时间点呈明显增加趋势,且电针组各时间点rCBF均较模型组明显升高(P<0.05,0.01)。结论:电针能使脑缺血再灌注脑组织中EPO表达增加,明显提高局部脑组织血流量,有助于减轻脑缺血再灌注后脑组织损伤。Objective：To observe the influence of electroacupuncture therapy on cortex regional cerebral blood flow （rCBF） and EPO expression of SD rats after cerebral ischemia reperfusion.Methods：Eighty SD rats were randomly divided into normal group （n =10）,sham-operation group （n =10）,model group （n =30） and electroacupuncture group （EA group） （n=30）.The later two groups were further divided into 24-h,48-h and 72-h subgroups respectively,with 10 cases each.The model of focal cerebral ischemia reperfusion was established by inserting nylon thread into the middle cerebral artery occlusion （MCAO） and reperfusion except control group and sham-operation group in which the artery was separated only.EA group was treated with electroacupuncture therapy.The expression of EPO was detected by using immunohistochemistry and Western blotting.The rCBF was measured using a Doppler laser blood stream detector.Results：The expression of EPO in model group and EA group was increased at 24 h,peaked at 48 h,and decreased at 72 h,and that in EA group was significantly increased as compared with that in in model group at the 3 time points （P＜0.05 or 0.01）.The rCBF in model group and EA group was consecutively increased at the three time points,and that in EA group significantly increased as compared with that in model group at the 3 time points （P＜0.05 or 0.01）.Conclusion：Electroacupuncture in rats can promote the expression of EPO and increase the rCBF in ischemic brain tissue after focal cerebral ischemia reperfusion,which may be helpful to alleviate the damage of brain tissue.

关 键 词：脑缺血再灌注 电针 促红细胞生成素 脑血流量 

分 类 号：R245[医药卫生—针灸推拿学]