电针结合磁刺激对脊髓损伤大鼠逼尿肌兴奋性及α-SMA的影响  

Effect of electroacupuncture combined with magnetic stimulation therapy on the excitability of detrusor and expression ofα-SMA in rats with spinal cord injury

作　　者：程宇核[1] 王俊华[1] 李海峰[1] 朱小虎[1] 谢谨[1,2] 邹旭丹[1] 万超[1] 

机构地区：[1]湖北医药学院附属太和医院,湖北十堰442000 [2]湖北中医药大学,武汉430065

出　　处：《中国康复》2013年第5期327-329,共3页Chinese Journal of Rehabilitation

基　　金：湖北省教育厅重点科研立项(D20092104)

摘　　要：目的:探讨电针及磁刺激治疗脊髓损伤(SCI)模型大鼠,对其逼尿肌兴奋性及α-平滑肌肌动蛋白(α-SMA)的影响,从而探讨其作用机制。方法:SD大鼠50只,随机分为正常组、模型组、电针组、磁刺激组和联合组,分别采用对应的治疗;采用重物坠落打击方法制备SCI模型;治疗后,测定各组大鼠离体逼尿肌肌条的最小收缩张力及收缩频率,采用免疫组化法观察大鼠逼尿肌中α-SMA阳性细胞数量。结果:治疗10次后,膀胱逼尿肌离体肌条最小收缩张力比较,联合组明显低于模型组、磁刺激组和电针组,电针组和磁刺激组均明显低于模型组(均P<0.05);各组肌条收缩频率及α-SMA阳性细胞表达比较,联合组均明显高于模型组、磁刺激组和电针组,电针组和磁刺激组均明显高于模型组(均P<0.05)。结论:电针及磁刺激治疗均能显著提高脊髓损伤后膀胱逼尿肌中α-SMA含量,增强膀胱逼尿肌兴奋性,改善脊髓损伤后尿潴留状况,电针结合磁刺激治疗效果更好。Objective：To investigate the influence of electroacupuncture and magnetic stimulation therapy on the detrusor excitability and a-SMA of rats with spinal cord injury （SCI） and the mechanism.Methods：Forty SD rats were randomly divided into 5 groups：normal group,model group,electroacupuncture group,magnetic stimulation group,and combination group.The SCI model was prepared by heavy objects fall method.The minimum shrinkage tension and contraction frequency of isolated detrusor muscle strips in rats were measured.Immunohistochemical methods were adopted to quantify the α-SMA positive cells of the detrusor.Results：After 10 times of treatment,the minimum shrinkage tension in combination group was obviously lower than model group,electroacupuncture group and magnetic stimulation group,and that in electroacupuncture group and magnetic stimulation group was lower than in model group （all P＜0.05）.The contraction frequency and the number of α-SMA positive cells in combination group were obviously increased as compared with model group,electroacupuncture group and magnetic stimulation group,and those in electroacupuncture group and magnetic stimulation group were significantly increased as compared with model group （all P＜0.05）.Conclusion：Electroacupuncture and magnetic stimulation therapy could significantly increase α-SMA content of the detrusor and detrusor excitability,and improve urinary retention condition,and the combination of electroacupuncture with magnetic stimulation treatment showed more satisfactory effectiveness.

关 键 词：脊髓损伤 针刺 电针 磁刺激 尿潴留 Α-平滑肌肌动蛋白 

分 类 号：R245[医药卫生—针灸推拿学]